Application Data
(Recombinant Rat IL-2 stimulates cell proliferation of the HT?2 mouse T cells. The ED50 for this effect is 0.65?2.6 ng/mL, corresponding to a specific activity of 3.85 × 10<sup>5</sup>~1.54× 10<sup>6</sup> units/mg.)
IL-2 recombinant protein
Recombinant Rat IL-2 Protein
Purity
>97% by SDS-PAGE.
Synonyms
IL-2; N/A; Recombinant Rat IL-2 Protein; IL2; IL-2 recombinant protein
Host
HEK293 cells
Purity/Purification
>97% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH7.4.
Sequence
PTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ
Species
Rat
Tag
C-His
Endotoxin
<0.1EU/ug
Bio-Activity
Measured in a cell proliferation assay using HT?2 mouse T cells. The ED50 for this effect is 0.65?2.6ng/mL, corresponding to a specific activity of 3.85 × 105~1.54× 106 units/mg.
Preparation and Storage
Store at -20 degree C. Store the lyophilized protein at -20 degree C to -80 degree C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
Application Data
(Recombinant Rat IL-2 stimulates cell proliferation of the HT?2 mouse T cells. The ED50 for this effect is 0.65?2.6 ng/mL, corresponding to a specific activity of 3.85 × 10<sup>5</sup>~1.54× 10<sup>6</sup> units/mg.)
Application Data
(Recombinant Rat IL-2 stimulates cell proliferation of the HT?2 mouse T cells. The ED50 for this effect is 0.65?2.6 ng/mL, corresponding to a specific activity of 3.85 × 10<sup>5</sup>~1.54× 10<sup>6</sup> units/mg.)
SDS-PAGE
(Recombinant Rat IL-2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 14-18 kDa.)
SDS-PAGE
(Recombinant Rat IL-2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 14-18 kDa.)
Related Product Information for IL-2 recombinant protein
Interleukin-2, also known as a T-cell growth factor, TCGF, Aldesleukin, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2/IL-2 was the first interleukin molecule to be discovered. Interleukin-2/IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2/IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2/IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells). Interleukin-2/IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2/IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2/IL-2 and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2/IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Product Categories/Family for IL-2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
UniProt Protein Name
Interleukin-2
UniProt Gene Name
Il2
UniProt Synonym Gene Names
Il-2; IL-2; TCGF
UniProt Entry Name
IL2_RAT
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Product Notes
The IL-2 il2 (Catalog #AAA283364) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: PTSSPAKETQ QHLEQLLLDL QVLLRGIDNY KNLKLPMMLT FKFYLPKQAT ELKHLQCLEN ELGALQRVLD LTQSKSFHLE DAGNFISNIR VTVVKLKGSE NKFECQFDDE PATVVEFLRR WIAICQSIIS TMTQ. It is sometimes possible for the material contained within the vial of "IL-2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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