Standard Curve (Sample)
(Mouse IL-21 PicoKine ELISA Kit standard curve)
Mouse IL-21 ELISA Kit | IL21 elisa kit
Mouse IL-21 PicoKine ELISA Kit
Gene Names
Il21; IL-21
Reactivity
Mouse
Synonyms
IL-21; N/A; Mouse IL-21 PicoKine ELISA Kit; Interleukin-21; interleukin 21; Cytokine CX2; DN 452A22.7; IL 21; Interleukin 21; Interleukin 21 isoform; Interleukin21; OTTMUSP00000007838; Za11; IL21 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-21
Sequence Length
146
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse IL-21 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse IL-21 PicoKine ELISA Kit standard curve)
Related Product Information for IL21 elisa kit
Principle of the assay: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli,P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.
Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,811 Da
NCBI Official Full Name
Interleukin 21 (Il21 protein)
NCBI Official Synonym Full Names
interleukin 21
NCBI Official Symbol
Il21
NCBI Official Synonym Symbols
IL-21
NCBI Protein Information
interleukin-21
UniProt Protein Name
Interleukin
UniProt Gene Name
Il21
UniProt Entry Name
Q5SUE2_MOUSE
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Product Notes
The Mouse IL21 il21 (Catalog #AAA45565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45565 ELISA Kit recognizes Mouse IL21. It is sometimes possible for the material contained within the vial of "IL-21, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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