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Application Data
(The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.Expression of EBNA-2 and LMP-1 in IL-21 treated cells .)
Interleukin-21 Recombinant Protein | Il21 recombinant protein
Mouse Interleukin-21 Recombinant
Gene Names
Il21; IL-21
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-21; N/A; Mouse Interleukin-21 Recombinant; Il21 recombinant protein
Host
E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-21 was lyophilized from a 0.2 um filtered PBS solution pH7.5.
Sequence
MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS
Source
Optimized DNA sequence encoding Human Interleukin-21 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Cytokine
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Application Data
(The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.Expression of EBNA-2 and LMP-1 in IL-21 treated cells .)
Application Data
(The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.Expression of EBNA-2 and LMP-1 in IL-21 treated cells .)
Application Data
(The CD4+ T cell computational model predicts modulation of differentiation and maintenance by IL-21 in silico.Sensitivity analysis of IL-21 over Th1- and Th17-related molecules showing positive or negative correlation to IL-21.)
Application Data
(The CD4+ T cell computational model predicts modulation of differentiation and maintenance by IL-21 in silico.Sensitivity analysis of IL-21 over Th1- and Th17-related molecules showing positive or negative correlation to IL-21.)
Application Data
(TGF-? enhances the effect of IL-21 on the development of IL-21-producing CD4+ T cells.Naïve CD4+ T cells from CBMCs were stimulated for 3 days with immobilized anti-CD3 and anti-CD28 mAbs (neutral condition) in the presence or absence of IL-21, TGF-? or IL-21 plus TGF-?.)
Application Data
(TGF-? enhances the effect of IL-21 on the development of IL-21-producing CD4+ T cells.Naïve CD4+ T cells from CBMCs were stimulated for 3 days with immobilized anti-CD3 and anti-CD28 mAbs (neutral condition) in the presence or absence of IL-21, TGF-? or IL-21 plus TGF-?.)
Application Data
Application Data
Application Data
(Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.)
Application Data
(Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.)
Product Categories/Family for Il21 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,811 Da
NCBI Official Full Name
interleukin-21 isoform 2
NCBI Official Synonym Full Names
interleukin 21
NCBI Official Symbol
Il21
NCBI Official Synonym Symbols
IL-21
NCBI Protein Information
interleukin-21
UniProt Protein Name
Interleukin-21
UniProt Gene Name
Il21
UniProt Synonym Gene Names
IL-21
UniProt Entry Name
IL21_MOUSE
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Product Notes
The Il21 il21 (Catalog #AAA76401) is a Recombinant Protein produced from E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS. It is sometimes possible for the material contained within the vial of "Interleukin-21, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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