IL-21 R Recombinant Protein | IL-21R recombinant protein

Recombinant mouse IL-21 R protein

Cat. Num. AAA48412

• Gene Names: Il21r; NILR
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: IL-21 R; N/A; Recombinant mouse IL-21 R protein; Interleukin-21 receptor; IL-21 receptor; IL-21R; Il21r; Lymphocyte receptor beta; LR-beta; Novel interleukin receptor; Novel cytokine receptor 8; NR8; CD360; CD360 antigen; NILR; Nilr; IL-21R recombinant protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-237aa
• Sequence: CLDLTCYTDY LWTITCVLET RSPNPSILSL TWQDEYEELQ DQETFCSLHR SGHNTTHIWY TCHMRLSQFL SDEVFIVNVT DQSGNNSQEC GSFVLAESIK PAPPLNVTVA FSGRYDISWD SAYDEPSNYV LRGKLQYELQ YRNLRDPYAV RPVTKLISVD SRNVSLLPEE FHKDSSYQLQ VRAAPQPGTS FRGTWSEWSD PVIFQTQAGE PEAGWDPH

• Applicable Applications for IL-21R recombinant protein: Bioactivity, SDS-PAGE
• Species: Mouse
• Tag: hIgG-His-Tag
• Predicted Nterm: cys 20
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its ability to inhibit IFN-g secretion using NK-92 human natural killer cells. The ED50 range <= 4 ug/ml with mouse IL-2
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Mouse IL-21R in inhibit the IFN-g secretion of the NK-92 human natural killer cells in the presence of mouse IL-21. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for IL-21R recombinant protein

IL-21R (Interleukin 21 receptor), also known as NILR, belongs to the type I cytokine receptor family and Type 4 subfamily. This receptor is Heterodimer with the common gamma subunit and transduces the growth promoting signal of IL21. It is important for the proliferation and differentiation of T cells, B cells, and NK cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. B cell IL?21 R engagement induces Blimp-1, and is important for memory responses. It engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production. It is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen and IL21 impacts some cell types, including CD8+ memory T cells, NK cells, and subsets of CD4 memory T cells. It is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation. Recombinant mouse IL21R, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-21R recombinant protein

Immunology; Hormones & Cytokines

References

Parrish-Novak, et al. (2000) Nature 408:57-63;; Mol Cell Biol. (2005) Nov; 25(22): 9741-9752.;; De Totero D, et al. (2006) Blood. 107(9): 3708-3715.;; Kasaian, M.T. et al. (2002) Immunity 16:559-569.;;

NCBI and Uniprot Product Information

• NCBI GI #: 11230786
• NCBI GeneID: 60504
• NCBI Accession #: NP_068687.1
• NCBI GenBank Nucleotide #: NM_021887.2
• UniProt Accession #: Q9JHX3; Q9ESM1
• Molecular Weight: 58,355 Da
• NCBI Official Full Name: interleukin-21 receptor
• NCBI Official Synonym Full Names: interleukin 21 receptor
• NCBI Official Symbol: Il21r
• NCBI Official Synonym Symbols: NILR
• NCBI Protein Information: interleukin-21 receptor; NR8; IL-21R; LR-beta; IL-21 receptor; lymphocyte receptor beta; novel cytokine receptor 8; novel interleukin receptor
• UniProt Protein Name: Interleukin-21 receptor
• UniProt Gene Name: Il21r
• UniProt Synonym Gene Names: Nilr; IL-21 receptor; IL-21R; LR-beta; NR8
• UniProt Entry Name: IL21R_MOUSE