Mouse IL-27 p28 ELISA Kit | IL27 elisa kit

Mouse IL-27 p28 PicoKine ELISA Kit

Cat. Num. AAA11590

• Gene Names: Il27; p28; Il30; IL-27; IL-27p28
• Reactivity: Mouse
• Synonyms: IL-27 p28; N/A; Mouse IL-27 p28 PicoKine ELISA Kit; Interleukin-27 subunit alpha; interleukin 27; IL 27A; IL 30; IL-27 subunit alpha; IL-27-A; IL27; IL27-A; IL27A; IL27A_HUMAN; IL27p28; IL30; Interleukin 27; Interleukin 27 subunit alpha; Interleukin 30; p28; IL27 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il27
• Sequence Length: 234
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for IL27 elisa kit

Principle of the Assay: The Picokine™ Mouse Il27 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il27 with a 96-well strip plate that is pre-coated with antibody specific for Il27. The detection antibody is a biotinylated antibody specific for Il27. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il27 with immunogen: Expression system for standard: NS0; Immunogen sequence: F29-S234. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il27, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Il27 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il27 in the sample.

Background/Introduction: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and Tlymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.

NCBI and Uniprot Product Information

• NCBI GI #: 81866680
• NCBI GeneID: 246779
• UniProt Accession #: Q8K3I6
• Molecular Weight: 26,542 Da
• NCBI Official Full Name: Interleukin-27 subunit alpha
• NCBI Official Synonym Full Names: interleukin 27
• NCBI Official Symbol: Il27
• NCBI Official Synonym Symbols: p28; Il30; IL-27; IL-27p28
• NCBI Protein Information: interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha
• UniProt Protein Name: Interleukin-27 subunit alpha
• UniProt Gene Name: Il27
• UniProt Synonym Gene Names: Il27a; IL-27 subunit alpha; IL-27-A; IL27-A
• UniProt Entry Name: IL27A_MOUSE