Rat interleukin 6 (interferon, beta 2) ELISA Kit | IL6 elisa kit
Rat Interleukin 6, IL-6 ELISA Kit
Gene Names
IL6; IL-6
Reactivity
Rat
Synonyms
interleukin 6 (interferon, beta 2); N/A; Rat Interleukin 6, IL-6 ELISA Kit; Rat Interleukin 6; IL-6 ELISA KIT; BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2; B-cell differentiation factor; CTL differentiation factor; OTTHUMP00000158544; hybridoma growth factor; interleukin 6; interleukin BSF-2; interleukin 6 (interferon; beta 2); IL6 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat IL-6. No significant cross-reactivity or interference between rat IL-6 and analogues was observed.
Sequence Length
241
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.312 pg/ml-20 pg/ml
Sensitivity
< 0.078 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for IL6 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,021 Da
NCBI Official Full Name
interleukin-6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
IL-6
NCBI Protein Information
interleukin-6; interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IL-6
UniProt Entry Name
IL6_RABIT