Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer Recombinant Protein

Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer Protein (His Tag)(HPLC-verified)

Cat. Num. AAA259242

• Purity: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.; = 90% as determined by SEC-MALS.
• Synonyms: Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer; N/A; Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer Protein (His Tag)(HPLC-verified); Harvey rat sarcoma viral oncogene homolog; Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.; = 90% as determined by SEC-MALS.
• Form/Format: Lyophilized from sterile 25mM Sodium Citrate, 200mM NaCl, pH 6.0.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: H3N2
• Predicted N Terminal: Gln 17
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Influenza A virus (A/Hong Kong/45/2019 (H3N2)) hemagglutinin (translated amino acids of EPI1588464), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
• Activity: 1.Immobilized Recombinant Influenza A H3N2 (A/Hong Kong/45/2019)HA trimer protein (His Tag) at 2 ug/mL (100 uL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 12-36 ng/mL.; 2.Recombinant Influenza A H3N2 (A/Hong Kong/45/2019)HA trimer Protein, His Tag, HPLC-verified was more than 95% and the molecular weight of this protein is around 245.3 kDa verified by SEC-MALS (Routinely tested).
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

(Recombinant Influenza A H3N2 (A/Hong Kong/45/2019)HA trimer Protein, His Tag, HPLC-verified(#AAA259242)was more than 95% and the molecular weight of this protein is around 245.3 kDa verified by SEC-MALS(Routinely tested).)

Application Data

(Immobilized Recombinant Influenza A H3N2 (A/Hong Kong/45/2019)HA trimer protein (His Tag) (#AAA259242) at 2 ug/mL (100 uL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 12-36 ng/mL.)

Application Data

Application Data

Related Product Information for Influenza A H3N2 (A/Hong Kong/45/2019) Hemagglutinin/HA trimer recombinant protein

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant hemagglutinin of the Influenza A virus (A/Hong Kong/45/2019 (H3N2)) consists of 558 amino acids and predicts a molecular mass of 62.55 kDa. It migrates as an approximately 91.6 kDa band in SDS-PAGE under reducing conditions.