Insulin ELISA Kit | INS elisa kit

Human/Canine/Porcine Insulin ELISA Kit PicoKine

Cat. Num. AAA128038

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Reactivity: Human, Canine, Porcine
• Applications: ELISA
• Synonyms: Insulin; N/A; Human/Canine/Porcine Insulin ELISA Kit PicoKine; INS elisa kit

• Reactivity: Human, Canine, Porcine
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 15.6pmol/L-1,000pmol/L
• Sensitivity: <2pmol/L
• Capture antibody and Detection antibody: polyclonal antibody from goat; biotinylated polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: F25-N110
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for INS elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 1 pmol/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pmol/L)	0	15.6	31.2	62.5	125	250	500	1,000
  O.D.	0.046	0.084	0.197	0.281	0.461	0.888	1.251	2.012

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pmol/L)	28	93	474	25	118	274
  Standard deviation	1.53	2.72	10.57	1.14	5.17	21.81
  CV(%)	5.5%	2.9%	2.2%	4.5%	4.4%	8%

• Reproducibility:

  Lots	Lot 1 (pmol/L)	Lot 2 (pmol/L)	Lot 3 (pmol/L)	Lot 4 (pmol/L)	Mean (pmol/L)	Standard Deviation	CV (%)
  Sample 1	28	18	24	19	22	1.69	7.6%
  Sample 2	93	93	112	85	96	6.96	7.3%
  Sample 3	474	411	299	444	407	21.08	5.2%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human/Canine/Porcine Insulin ELISA Kit PicoKine standard curve)

Related Product Information for INS elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for INS elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin
• UniProt Protein Name: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN