Standard Curve (Sample)
Rat Insulin ELISA Kit | INS elisa kit
Rat Insulin ELISA Kit
Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
Reactivity
Rat
Synonyms
Insulin; Rat Insulin ELISA Kit; Insulin/INS; INS elisa kit
Reactivity
Rat
Specificity
Specifically recognize INS, no obvious cross reaction with other analogues
Sequence Length
110
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for INS elisa kit
Background: Insulin (INS) is a protein hormone produced by the beta cells of the pancreas. It plays a crucial role in regulating glucose metabolism. INS lowers blood glucose levels by promoting the uptake and utilization of glucose by cells, and by promoting the storage of glucose in the liver and muscles. It also promotes the synthesis of proteins and fats, and inhibits the breakdown of proteins and fats, to maintain a balance of nutrients within the body. Insufficient levels of INS can lead to diabetes. Type 1 diabetes is caused by an absolute deficiency of insulin, usually due to the immune system attacking and destroying beta cells in the pancreas. Type 2 diabetes, on the other hand, is caused by insufficient insulin or insulin resistance, and is often related to obesity, lifestyle, and genetic factors.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti INS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti INS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with INS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of INS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti INS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti INS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with INS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of INS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,537 Da
NCBI Official Full Name
insulin
NCBI Official Synonym Full Names
insulin
NCBI Official Symbol
INS
NCBI Official Synonym Symbols
IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
NCBI Protein Information
insulin
UniProt Protein Name
Insulin
UniProt Gene Name
INS
UniProt Entry Name
INS_HUMAN
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Product Notes
The Rat INS ins (Catalog #AAA17361) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17361 ELISA Kit recognizes Rat INS. It is sometimes possible for the material contained within the vial of "Insulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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