Insulin Recombinant Protein | INS recombinant protein
Human Insulin recombinant
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
Insulin; N/A; Human Insulin recombinant; insulin; INS recombinant protein
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Insulin was lyophilized from a 0.2 um filtered solution with no additives.
Sequence Length
110
Host Note
Optimized DNA sequence encoding Human Insulin mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
Centrifuge vial to ensure maximal prodict extraction, recommended 20s , 5K RPM.
Follow by reconstitution in distilled water to a concentration not less than 0.1mg/mL. If further dilutions are required it is recommended to dilute product in the formulation buffer as given in the presentation.
Follow by reconstitution in distilled water to a concentration not less than 0.1mg/mL. If further dilutions are required it is recommended to dilute product in the formulation buffer as given in the presentation.
Molecular Weight Note
Recombinant Insulin is a protein consisting of 51 amino acid residue subunits, and migrates as an approximateley 6 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Biological Activity
Activity was determined to be 30 IU/mg.
Preparation and Storage
Store lyophilized product at room temperature for 3 weeks or at -20°C for 12 months .
Store reconstituted prouct at 2-8°C for 3 weeks or at -20°C for 12 months.
*For long term storage, lab recommends the addition of a carier protein at 0.1%.
*Avoid repeated freeze/thaw cycles.
Store reconstituted prouct at 2-8°C for 3 weeks or at -20°C for 12 months.
*For long term storage, lab recommends the addition of a carier protein at 0.1%.
*Avoid repeated freeze/thaw cycles.
Product Categories/Family for INS recombinant protein
References
• the endosymbiont wolbachia increases Insulin/igf-like signalling in drosophila .Proc R Soc B, Nov 2009; 276: 3799 - 3807.
• mutant huntingtin interacts with β-tubulin and disrupts vesicular transport and Insulin secretion .Hum. Mol. Genet., Oct 2009; 18: 3942 - 3954.
• Insulin is a stronger inducer of Insulin resistance than hyperglycemia in mice with type 1 diabetes mellitus (t1dm) .J. Biol. Chem., Oct 2009; 284: 27090 - 27100.
• food Insulin index: physiologic basis for predicting Insulin demand evoked by composite meals .Am. J. Clinical Nutrition, Oct 2009; 90: 986 - 992.
• dietary protein during gestation affects maternal Insulin-like growth factor, Insulin-like growth factor binding protein, leptin concentrations, and fetal growth in heifers .J Anim Sci, Oct 2009; 87: 3304 - 3316.
• mutant huntingtin interacts with β-tubulin and disrupts vesicular transport and Insulin secretion .Hum. Mol. Genet., Oct 2009; 18: 3942 - 3954.
• Insulin is a stronger inducer of Insulin resistance than hyperglycemia in mice with type 1 diabetes mellitus (t1dm) .J. Biol. Chem., Oct 2009; 284: 27090 - 27100.
• food Insulin index: physiologic basis for predicting Insulin demand evoked by composite meals .Am. J. Clinical Nutrition, Oct 2009; 90: 986 - 992.
• dietary protein during gestation affects maternal Insulin-like growth factor, Insulin-like growth factor binding protein, leptin concentrations, and fetal growth in heifers .J Anim Sci, Oct 2009; 87: 3304 - 3316.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
insulin preproprotein
UniProt Protein Name
Insulin
UniProt Gene Name
INS
UniProt Entry Name
INS_HUMAN
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Product Notes
The INS ins (Catalog #AAA76102) is a Recombinant Protein produced from E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Insulin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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