Insulin Recombinant Protein | INS recombinant protein

Insulin Protein, Human, Recombinant

Cat. Num. AAA257434

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: Insulin; N/A; Insulin Protein, Human, Recombinant; Human Insulin/INS Protein; insulin; IDDM Protein, Human; IDDM1 Protein, Human; IDDM2 Protein, Human; ILPR Protein, Human; INS Protein, Human; IRDN Protein, Human; MODY10 Protein, Human; INS recombinant protein

• Host: Yeast
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 53aa
• Sequence: Phe1-Lys29 & Gly1-Asn21

• Species: Human
• Predicted N Terminal: Phe
• Protein Construction: |A DNA sequence encoding human insulin B chain (P01308) (Phe25-Lys53) and human insulin A chain (P01308) (Gly90-Asn110) was expressed with a linker AAK
• Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.2-1ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 ug/mL.)

Application Data

Related Product Information for INS recombinant protein

INS (Insulin) is a Protein Coding gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. The binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Diseases associated with INS include Hyperproinsulinemia and Maturity-Onset Diabetes Of The Young, Type 10. A multitude of mutant alleles with phenotypic effects has been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes mellitus, maturity-onset diabetes of the young type 10, and hyperproinsulinemia.

Product Categories/Family for INS recombinant protein

AMPK Signaling Pathway

References

Veedfald S, Plamboeck A, Deacon C F, et al. Cephalic phase secretion of insulin and other enteropancreatic hormones in humans[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2016, 310(1): G43-G51.

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin
• UniProt Protein Name: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN