Application Data
(Figure 3.FLT-1 Sandwich-ELISA using recombinant human soluble FLT-1as standard [AAA14952]. Mouse anti-human FLT-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection.)
VEGFR-1/Flt-1 (native), soluble Active Protein | VEGFR-1/Flt-1 active protein
Human VEGFR-1/Flt-1 (native), soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Purity
> 95% by SDS-PAGE
Synonyms
VEGFR-1/Flt-1 (native), soluble; N/A; Human VEGFR-1/Flt-1 (native), soluble; Recombinant Human Endogenous Soluble VEGFR-1/Flt-1; Fms-like tyrosine kinase 1; Vascular permeability factor receptor; VEGFR-1/Flt-1 active protein
Host
Insect Cells
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Lyophilized
Sequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEITIRGEHCNKKAVFSRISKFKSTRNDCTTQSNVKH
Sequence Length
661
Buffer
PBS
Length (aa)
661
Result by N-terminal sequencing
SKLKD
Reconstitution
The lyophilized sVEGFR-1 is soluble in water and most aqueous buffers and should be reconstituted in PBS to a concentration not lower than 100 ug/ml.
Biological Activity
The activity of sVEGFR-1 was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
Preparation and Storage
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-1 should be stored in working aliquots at -70°C. Avoid repeated freeze and thaw cycles.
Application Data
(Figure 3.FLT-1 Sandwich-ELISA using recombinant human soluble FLT-1as standard [AAA14952]. Mouse anti-human FLT-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection.)
Application Data
(Figure 3.FLT-1 Sandwich-ELISA using recombinant human soluble FLT-1as standard [AAA14952]. Mouse anti-human FLT-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection.)
Application Data
(Fig. 2: Inhibition of the VEGF165-induced proliferation in HUVECs by soluble VEGFR-1/Flt-1.VEGF165(10ng/ml) was preincubated with increasing amounts of sVEGFR-1/Flt-1 for 1h and then added to the cells.)
Application Data
(Fig. 2: Inhibition of the VEGF165-induced proliferation in HUVECs by soluble VEGFR-1/Flt-1.VEGF165(10ng/ml) was preincubated with increasing amounts of sVEGFR-1/Flt-1 for 1h and then added to the cells.)
SDS-PAGE
(Fig. 1: SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie stain .)
SDS-PAGE
(Fig. 1: SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie stain .)
Related Product Information for VEGFR-1/Flt-1 active protein
Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) is the naturally occurring form and was cloned from total RNA of human umbilical vein endothelial cells. The recombinant mature sVEGFR-1 is a glycosylated monomeric protein with a mass of approximately 96 kDa. The soluble receptor protein consists of the first 6 extracellular domains (Met1-His688) containing the unique 31 amino acids residues at the C-terminus. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly, a naturally occurring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis binding VEGF with the same affinity as the full-length receptor.
Product Categories/Family for VEGFR-1/Flt-1 active protein
References
1. Barleon et al., 1997, J Biol Chem 272:10382-8 2. Röckl et al., 1998, Exp Cell Res, 241: 161-170].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96 kDa (Monomer)
NCBI Official Full Name
vascular endothelial growth factor receptor 1 isoform 2
NCBI Official Synonym Full Names
fms-related tyrosine kinase 1
NCBI Official Symbol
FLT1
NCBI Official Synonym Symbols
FLT; FLT-1; VEGFR1; VEGFR-1
NCBI Protein Information
vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
UniProt Protein Name
Vascular endothelial growth factor receptor 1
UniProt Gene Name
FLT1
UniProt Synonym Gene Names
FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
UniProt Entry Name
VGFR1_HUMAN
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Product Notes
The VEGFR-1/Flt-1 flt1 (Catalog #AAA14952) is an Active Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHKQQVLETV AGKRSYRLSM KVKAFPSPEV VWLKDGLPAT EKSARYLTRG YSLIIKDVTE EDAGNYTILL SIKQSNVFKN LTATLIVNVK PQIYEKAVSS FPDPALYPLG SRQILTCTAY GIPQPTIKWF WHPCNHNHSE ARCDFCSNNE ESFILDADSN MGNRIESITQ RMAIIEGKNK MASTLVVADS RISGIYICIA SNKVGTVGRN ISFYITDVPN GFHVNLEKMP TEGEDLKLSC TVNKFLYRDV TWILLRTVNN RTMHYSISKQ KMAITKEHSI TLNLTIMNVS LQDSGTYACR ARNVYTGEEI LQKKEITIRG EHCNKKAVFS RISKFKSTRN DCTTQSNVKH. It is sometimes possible for the material contained within the vial of "VEGFR-1/Flt-1 (native), soluble, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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