Mouse anti-Mouse VEGFR-1/Flt-1 Monoclonal Antibody | anti-VEGFR-1/Flt-1 antibody

Mouse Anti-Human VEGFR-1/Flt-1

Cat. Num. AAA79085

• Gene Names: FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
• Reactivity: Mouse
• Applications: Immunoprecipitation, Western Blot, ELISA
• Purity: Protein G purified
• Synonyms: VEGFR-1/Flt-1, Antibody; Mouse Anti-Human VEGFR-1/Flt-1; Anti-human VEGFR-1/Flt-1 (#EWI); Fms-like tyrosine kinase 1; Vascular permeability factor receptor; anti-VEGFR-1/Flt-1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: IgG1; EWI
• Specificity: The antibody will detect native human VEGFR-1/Flt-1 in ELISA experiments, WB, IF and IP.
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 687

• Applicable Applications for anti-VEGFR-1/Flt-1 antibody: IP (Immunoprecipitation), WB (Western Blot), ELISA
• Immunogen: recombinant human soluble Flt-1 (D5) (RT# S01-012)
• Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 (D5) as the immunizing antigen
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

WB (Western Blot)

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Related Product Information for anti-VEGFR-1/Flt-1 antibody

Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) is the naturally occurring form and was cloned from total RNA of human umbilical vein endothelial cells. The mature rh-sVEGFR-1 is a glycosylated monomeric protein with a mass of approximately 96kDa. The soluble receptor consists of the first 6 extracellular domains containing the unique 31 amino acids residues at the C-terminus.Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis binding VEGF with the same affinity as the full-length receptor.

References

1. Barleon et al., 1997, J Biol Chem 272:10382-8 2. Röckl et al., 1998, Exp Cell Res, 241: 161-170].

NCBI and Uniprot Product Information

• NCBI GI #: 229892220
• NCBI GeneID: 2321
• NCBI Accession #: NP_001153392
• NCBI GenBank Nucleotide #: NM_0001159920
• UniProt Accession #: P17948; O60722; A3E342; A3E344; A8KA71; B0LPF1; B2BF46; B2BF47; B2BF48; B3FR89; B5A923; F5H5L6
• Molecular Weight: 150,769 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1 isoform 2
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 1
• NCBI Official Symbol: FLT1
• NCBI Official Synonym Symbols: FLT; FLT-1; VEGFR1; VEGFR-1
• NCBI Protein Information: vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: FLT1
• UniProt Synonym Gene Names: FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
• UniProt Entry Name: VGFR1_HUMAN