<
Application Data
(Flt-1 Sandwich-ELISA using recombinant human sFlt1-14 as standard.Mouse anti-human Flt-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human Flt-1 was used for detection.)
VEGFR-1/Flt1-14 active protein
Human VEGFR-1/Flt1-14, soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Purity
> 95% by SDS-PAGE & Coomassie stain
Synonyms
VEGFR-1/Flt1-14; N/A; Human VEGFR-1/Flt1-14, soluble; Fms-like tyrosine kinase 1, Vascular permeability factor receptor, sFlt1-14 ; VEGFR-1/Flt1-14 active protein
Host
Insect cells
Purity/Purification
> 95% by SDS-PAGE & Coomassie stain
Form/Format
Lyophilized
Sequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEAPYLLRNLSDHTVAISSSTTLDCHANGVPEPQITWFKNNHKIQQEPELYTSTSPSSSSSSPLSSSSSSSSSSSS
Sequence Length
707
Application Notes
Optimal dilutions should be determined by each laboratory for each application.
Buffer
PBS
Reconstitution
The lyophilized sFlt1-14 is soluble in water and most aqueous buffers and should be reconstituted in water to a concentration not lower than 100 ug/ml
Length (aa)
707
Protein RefSeq
NP_001153502.1
mRNA RefSeq
NM_001160030.1
Stabilizer
None
Result by N terminal sequencing
SKLKD
Biological Activity
The activity of sFlt1-14 was determined by its ability to inhibit the VEGF165-induced proliferation of HDLECs.
Preparation and Storage
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sFlt1-14 should be stored in working aliquots at -70°C.
Avoid repeated freeze-thaw cycles!
Avoid repeated freeze-thaw cycles!
Application Data
(Flt-1 Sandwich-ELISA using recombinant human sFlt1-14 as standard.Mouse anti-human Flt-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human Flt-1 was used for detection.)
Application Data
(Flt-1 Sandwich-ELISA using recombinant human sFlt1-14 as standard.Mouse anti-human Flt-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human Flt-1 was used for detection.)
Application Data
(Inhibition of VEGF165-induced proliferation in HDLECs by sFlt1-14. VEGF165 (10ng/ml) was preincubated with increasing amounts of soluble receptor for 1h respectively and then added to the cells.)
Application Data
(Inhibition of VEGF165-induced proliferation in HDLECs by sFlt1-14. VEGF165 (10ng/ml) was preincubated with increasing amounts of soluble receptor for 1h respectively and then added to the cells.)
Application Data
(VEGF-A BioLISA using recombinant human soluble Flt1-14 for capturing and various recombinant human VEGF-A isoforms as standard. A Biotin-conjugated rabbit anti-human VEGF-A antibody [Cat# ] was used for detection. As expected also mouse VEGF164 binds to sFlt1-14)
Application Data
(VEGF-A BioLISA using recombinant human soluble Flt1-14 for capturing and various recombinant human VEGF-A isoforms as standard. A Biotin-conjugated rabbit anti-human VEGF-A antibody [Cat# ] was used for detection. As expected also mouse VEGF164 binds to sFlt1-14)
SDS-PAGE
(SDS-PAGE analysis of recombinant human sFlt1-14 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
SDS-PAGE
(SDS-PAGE analysis of recombinant human sFlt1-14 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
Related Product Information for VEGFR-1/Flt1-14 active protein
A human-specific splicing variant of vascular endothelial growth factor (VEGF) receptor 1 (Flt1) was discovered, producing a soluble receptor (designated sFlt1-14) that is qualitatively different from the previously described soluble receptor (sFlt1) and functioning as a potent VEGF inhibitor. sFlt1-14 is generated in a cell type-specific fashion, primarily in non-endothelial cells. Notably, in vascular smooth muscle cells, all Flt1 messenger RNA is converted to sFlt1-14, whereas endothelial cells of the same human vessel express sFlt1. sFlt1-14 expression by vascular smooth muscle cells is dynamically regulated as evidenced by its upregulation on coculture with endothelial cells or by direct exposure to VEGF. Increased production of soluble VEGF receptors during pregnancy is entirely attributable to induced expression of placental sFlt1-14 starting by the end of the first trimester. Expression is dramatically elevated in the placenta of women with preeclampsia, specifically induced in abnormal clusters of degenerative syncytiotrophoblasts known as syncytial knots, where it may undergo further messenger RNA editing. sFlt1-14 is the predominant VEGF-inhibiting protein produced by the preeclamptic placenta, accumulates in the circulation, and hence is capable of neutralizing VEGF in distant organs affected in preeclampsia. Together, these findings revealed a new natural VEGF inhibitor that has evolved in humans, possibly to protect non-endothelial cells from adverse VEGF signaling. Furthermore, the study uncovered the identity of a VEGF-blocking protein implicated in preeclampsia.
Product Categories/Family for VEGFR-1/Flt1-14 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~105 kDa (Monomer)
NCBI Official Full Name
vascular endothelial growth factor receptor 1 isoform 2
NCBI Official Synonym Full Names
fms related tyrosine kinase 1
NCBI Official Symbol
FLT1
NCBI Official Synonym Symbols
FLT; FLT-1; VEGFR1; VEGFR-1
NCBI Protein Information
vascular endothelial growth factor receptor 1
UniProt Protein Name
Vascular endothelial growth factor receptor 1
UniProt Gene Name
FLT1
UniProt Synonym Gene Names
FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
UniProt Entry Name
VGFR1_HUMAN
Similar Products
Product Notes
The VEGFR-1/Flt1-14 flt1 (Catalog #AAA14956) is an Active Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the VEGFR-1/Flt1-14 flt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHRKQQVLET VAGKRSYRLS MKVKAFPSPE VVWLKDGLPA TEKSARYLTR GYSLIIKDVT EEDAGNYTIL LSIKQSNVFK NLTATLIVNV KPQIYEKAVS SFPDPALYPL GSRQILTCTA YGIPQPTIKW FWHPCNHNHS EARCDFCSNN EESFILDADS NMGNRIESIT QRMAIIEGKN KMASTLVVAD SRISGIYICI ASNKVGTVGR NISFYITDVP NGFHVNLEKM PTEGEDLKLS CTVNKFLYRD VTWILLRTVN NRTMHYSISK QKMAITKEHS ITLNLTIMNV SLQDSGTYAC RARNVYTGEE ILQKKEITIR DQEAPYLLRN LSDHTVAISS STTLDCHANG VPEPQITWFK NNHKIQQEPE LYTSTSPSSS SSSPLSSSSS SSSSSSS. It is sometimes possible for the material contained within the vial of "VEGFR-1/Flt1-14, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.