Vascular Endothelial Growth Factor receptor-3 Active Protein | FLT4 active protein

Recombinant Human Vascular Endothelial Growth Factor receptor-3

Cat. Num. AAA38401

• Gene Names: FLT4; PCL; FLT41; LMPH1A; VEGFR3
• Purity: Greater than 90.0% as determined by(a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
• Synonyms: Vascular Endothelial Growth Factor receptor-3; N/A; Recombinant Human Vascular Endothelial Growth Factor receptor-3; FLT4 Human; Vascular Endothelial Growth Factor Receptor-3 Human Recombinant; Tyrosine-protein kinase receptor FLT4; PCL; FLT41; FMS-LIKE TYROSINE KINASE 4; VEGFR-3; VEGFR3; FLT4 active protein

• Host: Insect Cells
• Purity/Purification: Greater than 90.0% as determined by(a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
• Form/Format: FLT4 was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 1298

• Solubility: It is recommended to reconstitute the lyophilized FLT4 in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human VEGFR-3/FLT-4 at 5 ug/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml.
• Preparation and Storage: Lyophilized FLT4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FLT4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for FLT4 active protein

Description: Soluble FLT4 Human Recombinant fused with a carboxy-terminal 6X histidine-tag produced in baculovirus is a monomeric, glycosylated, polypeptide containing the extracellular part, 25-774 amino acids and having a total molecular mass of 120 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for ligand binding. The FLT4 is purified by proprietary chromatographic techniques.

Introduction: All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis.

Product Categories/Family for FLT4 active protein

Protein Kinases; Enzymes; VEGF Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 103472027
• NCBI GeneID: 2324
• NCBI Accession #: NP_002011.2
• NCBI GenBank Nucleotide #: NM_002020.4
• UniProt Accession #: P35916; Q16067; Q86W07; Q86W08; A8K6L4; B5A926; AAB23636
• Molecular Weight: 93,174 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 3 isoform 2
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 4
• NCBI Official Symbol: FLT4
• NCBI Official Synonym Symbols: PCL; FLT41; LMPH1A; VEGFR3
• NCBI Protein Information: vascular endothelial growth factor receptor 3; FLT-4; VEGFR-3; fms-like tyrosine kinase 4; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; tyrosine-protein kinase receptor FLT4
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• UniProt Gene Name: FLT4
• UniProt Synonym Gene Names: VEGFR3; VEGFR-3; FLT-4
• UniProt Entry Name: VGFR3_HUMAN