EGFR, soluble Active Protein | EGFR active protein

Human EGFR, soluble

Cat. Num. AAA79134

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61
• Reactivity: Human
• Purity: > 90% by SDS-PAGE
• Synonyms: EGFR, soluble; N/A; Human EGFR, soluble; Recombinant Human Soluble EGFR; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; EGFR active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Lyophilized
• Sequence: LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSWSHPQFEK
• Sequence Length: 629

• Biological Activity: Measured by its ability to bind to immobilized recombinant human EGF in a functional ELISA.
• Buffer: PBS
• Length (aa): 629
• Reconstitution: The lyophilized sEGFR is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50ug/ml.
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Reconstituted sEGFR should be stored in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles!

BioLISA

(Fig 2: Binding of sEGFRto recombinant human EGF in a functional ELISA. EGF [AAA79134] was coated with 200ng/well to a 96-well plate and sEGFR was added with increasing concentrations up to 4000ng/ml. Detection was performed using a polyclonal rabbit anti-human EGFR [] (1 ug/ml, 100 ul/well) and a goat anti-rabbit Biotin conjugated secondary antibody.)

SDS-PAGE

(Fig. 1: SDS-PAGE analysis of recombinant human soluble EGFR from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie Blue.)

Related Product Information for EGFR active protein

Recombinant human soluble EGFR, fused to a C-terminal Strep-tag, is produced as a glycosylated monomeric protein with a mass of approximately 80kDa in insect cells. The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoprotein that has an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24aa putative signal peptide, a 621aa extracellular domain, a 23aa transmembrane domain, and a 542aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis.

Product Categories/Family for EGFR active protein

Soluble receptors

References

1. Daly, R.J., Growth Factors, 16:255, 1999 2. Schlessinger, J., Cell. 103:211, 2000 3. Maihle, N.J. et al., Cancer Treat. Res. 107:247, 2002

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NM_005228.3
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• Molecular Weight: ~85 kDa
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61
• NCBI Protein Information: epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN