GM-CSF active protein

Human GM-CSF

Cat. Num. AAA79128

• Gene Names: CSF2; GMCSF
• Reactivity: Human
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: GM-CSF; N/A; Human GM-CSF; Recombinant Human Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF); Colony-stimulating factor; GM-CSF active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQ EPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQI ITFESFKENLKDFLLVIPFDCWEPVQETRHHHHHH
• Sequence Length: 135

• N Terminal Sequence: APARSPSPST
• Buffer: PBS
• Preparation and Storage: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20 degree C. Reconstituted GM-CSF should be stored in working aliquots at -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid repeated freeze-thaw cycles.

Application Data

Application Data

Related Product Information for GM-CSF active protein

Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14.5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific alpha chain and a common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Product Categories/Family for GM-CSF active protein

Cytokines & Growth Factors

References

1. Martinez-Moczygemba and Huston (2003) J. Allergy Clin Immunol 112:653 2. Barreda et al, (2004) Dev Comp Immunol 28:509 3. Eksioglu et al, (2007) Exp Hematol 35:1163 4. Cao Y, (2007) J Clin Invest 117:2362 5. Fleetwood et al, (2005) Crit Rev Immunol 25:405 6. Diederichs et al, (1991) Science 254:1779 7. Gough et al, (1984) Nature 309:763

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_0000749
• NCBI GenBank Nucleotide #: NM_000758
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: ~15.0 - 18.0 kDa
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN