Human Insulin-like 3 ELISA Kit | INSL3 elisa kit

Human Insulin-like 3 ELISA Kit

Cat. Num. AAA100922

• Gene Names: INSL3; RLF; RLNL; ley-I-L
• Reactivity: Human
• Synonyms: Insulin-like 3; N/A; Human Insulin-like 3 ELISA Kit; INSL3 (Insulin-like 3)/Ley I-L/RLFley-I-L/RLF/RLNL/insulin-like 3/insulin-like 3 (Leydig cell)/leydig insulin -like hormone/leydig insulin -like peptide/Relaxin-like factor/relaxin-like factor b; INSL3 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize INSL3, no obvious cross reaction with other analogues
• Sequence Length: 131
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for INSL3 elisa kit

Background: Insulin-like factor 3 (INSL3) is a peptide secreted by testicular Leydig cells and to a smaller extent by follicular theca cells. It acts via a G-protein-coupled receptor that is expressed in several organs, including bone, brain, kidney, muscle, epididymis, gubernaculum, and germ cells. It can also be used as a marker of Leydig cell function in fetal and perinatal period, which can help to identify whether a developing gonad is a testis when only the Leydig cells can produce it.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti INSL3 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti INSL3 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with INSL3 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of INSL3 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 317373369
• NCBI GeneID: 3640
• Molecular Weight: 16,872 Da
• NCBI Official Full Name: Insulin-like 3
• NCBI Official Synonym Full Names: insulin like 3
• NCBI Official Symbol: INSL3
• NCBI Official Synonym Symbols: RLF; RLNL; ley-I-L
• NCBI Protein Information: insulin-like 3
• UniProt Protein Name: Insulin-like 3
• UniProt Gene Name: INSL3
• UniProt Synonym Gene Names: RLF; RLNL; Ley-I-L
• UniProt Entry Name: INSL3_HUMAN