IRS1 recombinant protein

IRS1 recombinant protein

Cat. Num. AAA14249

• Gene Names: IRS1; HIRS-1
• Applications: SDS-Page
• Purity: >80%
• Synonyms: IRS1; N/A; IRS1 recombinant protein; Insulin Receptor Substrate 1; HIRS-1

• Host: Sf9 insect cells
• Purity/Purification: >80%
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2mg/mL (varies by lot)
• Sequence Positions: 600-1245
• Sequence Length: 8743

• Applicable Applications for IRS1 recombinant protein: SDS-PAGE
• Tag Information: N-terminal GST tag
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

(The purity of IRS 1 was determined to be >8O% by densitometry. Approx. MW 118kDa.)

Related Product Information for IRS1 recombinant protein

Recombinant human IRS1 (600-1245) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: IRS1 is the substrate for the insulin tyrosine kinase receptor and is found in a variety of insulin-responsive cells and tissues. IRS1 protein has no intrinsic enzymatic activity but acts as a docking protein, via the SH2 domains, for mediating the insulin downstream signaling events. IRS1 has been shown to associate with the 14-3-3 family of proteins and this could play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (1). IRS1 may be associated with colorectal cancer and diet and related factors may affect the risk by modifying plasma insulin levels. Thus, the inter-individual variation in insulin signaling mediated by IRS1 may play a plausible role in the development of colorectal cancer (2).

Product Categories/Family for IRS1 recombinant protein

Proteins Substrates; Signaling Reagents - Protein Substrates

References

1. Ogihara, T. et al: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J. Biol. Chem. 272: 25267-25274, 1997.
2. Slattery, M.L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in outhwestern United States. Breast Cancer Res Treat. 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 187761322
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544
• UniProt Accession #: P35568
• NCBI Official Full Name: Homo sapiens insulin receptor substrate 1 (IRS1), mRNA
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1; IRS-1
• UniProt Protein Name: Insulin receptor substrate 1
• UniProt Gene Name: IRS1
• UniProt Synonym Gene Names: IRS-1
• UniProt Entry Name: IRS1_HUMAN