ELISA
(Raw Data)
Purified IgY Isotype Control | IgY isotype control
Purified Chicken IgY
Applications
ELISA
Synonyms
Purified IgY; N/A; Purified Chicken IgY; Purified Chicken IgY h+l PROTEIN REFERENCE STANDARDS; Chicken Protein Reference Standards; IgY isotype control
Specificity
Antibody concentration was determine by extinction coefficient: OD. 1.32 at 280nM equals 1.0mg of IgY. Optimal working dilutions should be determined experimentally by the investigator.
Form/Format
Lyophilized; PBS, pH 7.2 with non-protein stabilizer.
Applicable Applications for IgY isotype control
Direct ELISA
Application Notes
Quality Control Testing:
Quality control testing by Direct ELISA utilizing the following parameters:
Step 1: (Action) Antigen @ 500 ng/well. Incubated 60 minutes. ; (Materials Used) Purified Chicken IgY
Step 2: (Action) Block plates for 30 minutes ; (Materials Used) 1% BSA
Step 3: (Action)HRP Conjugated Antibody. Incubated 60 minutes. ; (Materials Used) HRP Goat anti-Chicken IgY h+I ( GGHL-30P)
Step 4: (Action) Develop with TMB-Incubated 5 minutes ; (Materials Used) TMB Substrate
Step 5: (Action) Stop reaction ; (Materials Used) 0.3M H2SO4
Step 6: (Action) Read plates at OD450 ; ( Materials Used) Microplates Reader set @ OD450
Quality control testing by Direct ELISA utilizing the following parameters:
Step 1: (Action) Antigen @ 500 ng/well. Incubated 60 minutes. ; (Materials Used) Purified Chicken IgY
Step 2: (Action) Block plates for 30 minutes ; (Materials Used) 1% BSA
Step 3: (Action)HRP Conjugated Antibody. Incubated 60 minutes. ; (Materials Used) HRP Goat anti-Chicken IgY h+I ( GGHL-30P)
Step 4: (Action) Develop with TMB-Incubated 5 minutes ; (Materials Used) TMB Substrate
Step 5: (Action) Stop reaction ; (Materials Used) 0.3M H2SO4
Step 6: (Action) Read plates at OD450 ; ( Materials Used) Microplates Reader set @ OD450
Procedure
The IgY h+l was from egg yolks using a combination of procedures that include salt fractionation.
Preparation and Storage
Lyophilized material should be kept at 2-8°C until rehydrated and will remain stable for two years.
Rehydrate by adding 0.5ml of reagent quality water to the product vial. Store the liquid solution at 2-8°C for up to a week: Thereafter, aliquots should be prepared and store frozen. Stable for a minimum of 1 year at -20°C to -80°C. Multiple freeze-thaw cycles are not recommended.
Rehydrate by adding 0.5ml of reagent quality water to the product vial. Store the liquid solution at 2-8°C for up to a week: Thereafter, aliquots should be prepared and store frozen. Stable for a minimum of 1 year at -20°C to -80°C. Multiple freeze-thaw cycles are not recommended.
ELISA
(Raw Data)
ELISA
(Raw Data)
ELISA
(Raw Data)
ELISA
(Raw Data)
Product Categories/Family for IgY isotype control
Similar Products
Product Notes
The IgY (Catalog #AAA14460) is an Isotype Control and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Purified IgY can be used in a range of immunoassay formats including, but not limited to, Direct ELISA. Quality Control Testing: Quality control testing by Direct ELISA utilizing the following parameters: Step 1: (Action) Antigen @ 500 ng/well. Incubated 60 minutes. ; (Materials Used) Purified Chicken IgY Step 2: (Action) Block plates for 30 minutes ; (Materials Used) 1% BSA Step 3: (Action)HRP Conjugated Antibody. Incubated 60 minutes. ; (Materials Used) HRP Goat anti-Chicken IgY h+I ( GGHL-30P) Step 4: (Action) Develop with TMB-Incubated 5 minutes ; (Materials Used) TMB Substrate Step 5: (Action) Stop reaction ; (Materials Used) 0.3M H2SO4 Step 6: (Action) Read plates at OD450 ; ( Materials Used) Microplates Reader set @ OD450. Researchers should empirically determine the suitability of the IgY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Purified IgY, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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