ITGA2 recombinant protein

Human ITGA2 Protein, His Tag

Cat. Num. AAA47545

• Gene Names: ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• Purity: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: ITGA2; N/A; Human ITGA2 Protein, His Tag; Integrin alpha-2; Collagen receptor; GPIa; CD49b; ITGA2 recombinant protein

• Host: HEK293
• Purity/Purification: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

• Tag: C-6xHis tag
• Reconstitution: 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.; 2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 128.21 uL; 3. After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
• Molecular Characterzation: ITGA2(Tyr30-Thr1132) 6xHis tag
• Visual Inspection: Test: Lyophilization; Specification: Dried white powder; Result: Pass; ; Test: Vial label; Specification: Label, Aligned and complete; Result: Pass; ; Test: Bottle sealing (manual check); Specification: Sealed tight; Result: Pass
• Quantity: Test: Bradford Method; Specification: > Specifed quantity; Result: Pass
• Other Properties: Test: pH; Specification: 7.4 ± 0.5; Result: Pass
• Preparation and Storage: Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing).

SDS-PAGE

(Figure 1. Human ITGA2, His Tag on SDS-PAGE under reducing condition.)

Related Product Information for ITGA2 recombinant protein

Recombinant human ITGA2 Protein with C-terminal 6×His tag
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Product Categories/Family for ITGA2 recombinant protein

Bioactive Protein

NCBI and Uniprot Product Information

• NCBI GI #: 116295258
• NCBI GeneID: 3673
• NCBI Accession #: NP_002194.2
• NCBI GenBank Nucleotide #: NM_002203.3
• UniProt Accession #: P17301; Q14595
• Molecular Weight: The protein has a predicted molecular mass of 121.8 kDa after removal of the signal peptide. The apparent molecular mass of ITGA2-His is approximately 130-250 kDa due to glycosylation.
• NCBI Official Full Name: integrin alpha-2
• NCBI Official Synonym Full Names: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• NCBI Official Symbol: ITGA2
• NCBI Official Synonym Symbols: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• NCBI Protein Information: integrin alpha-2; collagen receptor; platelet antigen Br; platelet glycoprotein GPIa; VLA2 receptor, alpha-2 subunit; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; human platelet alloantigen system 5; very late activation protein 2
• UniProt Protein Name: Integrin alpha-2
• UniProt Gene Name: ITGA2
• UniProt Synonym Gene Names: CD49B; GPIa
• UniProt Entry Name: ITA2_HUMAN