Integrin alpha 4 beta 7 Recombinant Protein | ITGA4&ITGB7 recombinant protein

Recombinant Human Integrin alpha 4 beta 7 Protein (His Tag)

Cat. Num. AAA259394

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human
• Purity: >= 95% as determined by SDS-PAGE.
• Synonyms: Integrin alpha 4 beta 7; N/A; Recombinant Human Integrin alpha 4 beta 7 Protein (His Tag); Human Integrin alpha 4 beta 7 Heterodimer Protein (His Tag); ITGA4&ITGB7 recombinant protein

• Host: HEK293 Cells
• Reactivity: Human
• Purity/Purification: >= 95% as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence: Tyr34-Thr977 & Glu20-His723

• Tag: C-His
• Predicted N Terminal: Tyr34 & Glu20
• Endotoxin: < 1.0 EU perug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Human ITGA4 (P13612-1)(Tyr34-Thr977) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Human ITGB7 (P26010-1)(Glu20-His723) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the Human Integrin alpha 4 beta 7 Heterodimer was purified.
• Activity: 1. Immobilized Recombinant Human Integrin alpha 4 beta 7 Heterodimer Protein (His Tag) at 2ug/mL (100uL/well) can bind Anti-Integrin alpha4beta1 Antibody, Human IgG4 (natalizumab), the EC50 is 6-18ng/mL (Rountinely tested).; 2. Immobilized Anti-Integrin alpha4beta7 Antibody, Human IgG1 (Vedolizumab) at 2ug/mL (100uL/well) can bind Recombinant Human Integrin alpha 4 beta 7 Heterodimer Protein (DYKDDDDK & His Tag), the EC50 is 8-25ng/mL (QC tested).
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Application Data

(Immobilized Anti-Integrin alpha4beta7 Antibody, Human IgG1 (Vedolizumab) at 2ug/mL (100uL/well) can bind Recombinant Human Integrin alpha 4 beta 7 Heterodimer Protein (DYKDDDDK & His Tag)(#AAA259394), the EC50is 8-25ng/mL (QC tested).)

Application Data

(Immobilized Recombinant Human Integrin alpha 4 beta 7 Heterodimer Protein (His Tag)(#AAA259394) at 2ug/mL (100uL/well) can bind Anti-Integrin alpha4beta1 Antibody, Human IgG4 (natalizumab), the EC50 is 6-18ng/mL (Rountinely tested).)

Application Data

NCBI and Uniprot Product Information

• NCBI GI #: 311033436
• NCBI GeneID: 3676
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4; AAB25486
• Molecular Weight: 1032
• NCBI Official Full Name: Integrin alpha-4
• NCBI Official Synonym Full Names: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 sub
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN