Application Data
ITPRIPL1 recombinant protein
ITPRIPL1 Protein, Human, Recombinant (ECD, His Tag), HPLC-verified
Purity
>= 95% as determined by SDS-PAGE.= 90% as determined by SEC-HPLC.
Synonyms
ITPRIPL1; N/A; ITPRIPL1 Protein, Human, Recombinant (ECD, His Tag), HPLC-verified; inositol 1,4,5-trisphosphate receptor interacting protein-like 1; KIAA1754L Protein, Human; ITPRIPL1 recombinant protein
Host
HEK293 Cells
Purity/Purification
>= 95% as determined by SDS-PAGE.
= 90% as determined by SEC-HPLC.
= 90% as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Species
Human
Predicted N Terminal
His 33
Endotoxin
<1.0 EU per ug protein as determined by the LAL method.
Protein Construction
A DNA sequence encoding the Human ITPRIPL1 (AAH34503.2)(His33-Gly111) was expressed with a polyhistidine tag at the C-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for ITPRIPL1 recombinant protein
Inositol 1,4,5-trisphosphate Receptor Interacting Protein-Like 1 or ITPRIPL1 is a transmembrane protein with broad expression in the testis associated with immune evasion. In fact, ITPRIPL1 is a molecule specifically expressed in immune privileged tissues, "hot" tumors without PD-L1 expression, and non-responding tumors to PD-1 blockade therapy. ITPRIPL1 has been shown to impair T cell function in vitro and in vivo through CD3e with experimental evidence supporting a negative correlation between ITPRIPL1 expression level in antigen-presenting cells and T cell cytotoxicity. ITPRIPL1 was a hypermethylated-low expression gene in breast cancer and acted as an independent biomarker for the prognosis of lung adenocarcinoma by using bioinformatics methods. Thus, targeting the ITPRIPL1-CD3e axis, especially in PD-1-PD-L1 negative patients, is a promising therapeutic strategy to reduce immune evasion.
References
1. Shouyan Deng, et al. The ITPRIPL1- CD3epsilon axis: a novel immune checkpoint controlling T cells activation. bioRxiv, 2022. doi: 10.1101/2022.02.25.481189 2. Zhong, X. and Zhong, G. Prognostic biomarker identification and tumor classification in breast cancer patients by methylation and transcriptome analysis. FEBS Open Bio, 2021. doi: 10.1002/2211-5463.13211
NCBI and Uniprot Product Information
Molecular Weight
The recombinant Human ITPRIPL1 consists of 90 amino acids and predicts a molecular mass of 10.75 kDa. It migrates as an approximately 13.2 kDa band in SDS-PAGE under reducing conditions.
Similar Products
Product Notes
The ITPRIPL1 (Catalog #AAA259194) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ITPRIPL1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.