Human Jagged1 ELISA Kit | JAG1 elisa kit

Human Jagged1 ELISA Kit PicoKine

Cat. Num. AAA127976

• Gene Names: JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Jagged1; N/A; Human Jagged1 ELISA Kit PicoKine; JAG1 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 62.5pg/ml-4,000pg/ml
• Sensitivity: <30pg/ml
• Capture antibody and Detection antibody: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
• Immunogen: Expression system for standard: CHO; Immunogen sequence: S32-S1046
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for JAG1 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
  O.D.	0.049	0.072	0.129	0.218	0.421	0.775	1.478	2.079

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	90	185	1704	76	200	1687
  Standard deviation	2.79	12.91	48.39	4.92	10.12	69.34
  CV(%)	3.1%	7%	2.8%	6.5%	5.1%	4.1%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	90	96	75	67	82	4.44	5.4%
  Sample 2	185	143	239	169	184	12.6	6.9%
  Sample 3	1704	1270	1116	1693	1446	93.40	6.5%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human Jagged1 PicoKine ELISA Kit standard curve)

Related Product Information for JAG1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for JAG1 elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 4557679
• NCBI GeneID: 182
• NCBI Accession #: NP_000205
• NCBI GenBank Nucleotide #: NM_000214.2
• UniProt Accession #: P78504; O14902; O15122; Q15816; A0AV43; B4DYR1; E9PCF9
• Molecular Weight: Predicted: 134 kDa; ; Observed: 130 kDa
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: JAG1
• NCBI Official Synonym Symbols: AGS; AHD; AWS; HJ1; CD339; JAGL1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• UniProt Gene Name: JAG1
• UniProt Synonym Gene Names: JAGL1; Jagged1; hJ1
• UniProt Entry Name: JAG1_HUMAN