Application Data
(HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
JAK2 recombinant protein
Recombinant JAK2 (532-1132) protein
Gene Names
JAK2; JTK10; THCYT3
Synonyms
JAK2; N/A; Recombinant JAK2 (532-1132) protein; Janus Kinase 2; Tyrosine-Protein Kinase JAK2; EC 2.7.10; JAK2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant JAK2 (532-1132) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant JAK2 (532-1132) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
Application Data
(HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
Application Data
(HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
Application Data
(HTRF assay for Recombinant JAK2 (532-1132) protein activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity)
SDS-PAGE
(Recombinant JAK2 (532-1132) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 71 kDa Purity: >90%)
SDS-PAGE
(Recombinant JAK2 (532-1132) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 71 kDa Purity: >90%)
Related Product Information for JAK2 recombinant protein
Short Description: Recombinant JAK2 (532-1132) protein that includes amino acids 532-1132 of human JAK2 protein (accession number NP_004963.1) was expressed in a baculovirus expression system and contains an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 71 kDa. Recombinant JAK2 (532-1132) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: JAK2 (Janus Kinase 2), also known as Tyrosine-Protein Kinase JAK2 or JTK10, is a non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. In the cytoplasm, JAK2 plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO);or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, JAK2 can phosphorylate specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Then it can subsequently phosphorylate the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. JAK2 signaling is implicated in diverse biological processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and alteration of heterochromatin.
Background: JAK2 (Janus Kinase 2), also known as Tyrosine-Protein Kinase JAK2 or JTK10, is a non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. In the cytoplasm, JAK2 plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO);or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, JAK2 can phosphorylate specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Then it can subsequently phosphorylate the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. JAK2 signaling is implicated in diverse biological processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and alteration of heterochromatin.
Product Categories/Family for JAK2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,674 Da
NCBI Official Full Name
tyrosine-protein kinase JAK2
NCBI Official Synonym Full Names
Janus kinase 2
NCBI Official Symbol
JAK2
NCBI Official Synonym Symbols
JTK10; THCYT3
NCBI Protein Information
tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase)
UniProt Protein Name
Tyrosine-protein kinase JAK2
UniProt Gene Name
JAK2
UniProt Synonym Gene Names
JAK-2
UniProt Entry Name
JAK2_HUMAN
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Product Notes
The JAK2 jak2 (Catalog #AAA60212) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JAK2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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