Application Data
(Fig. Hela cells stained with AAA Biotech Mitochondrial Membrane Potential Assay Kit (JC-1).)
Mitochondrial Membrane Potential Assay Kit | JC-1 assay kit
Mitochondrial Membrane Potential Assay Kit (JC-1)
Synonyms
Mitochondrial Membrane Potential; N/A; Mitochondrial Membrane Potential Assay Kit (JC-1); JC-1 assay kit
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Application Data
(Fig. Hela cells stained with AAA Biotech Mitochondrial Membrane Potential Assay Kit (JC-1).)
Application Data
(Fig. Hela cells stained with AAA Biotech Mitochondrial Membrane Potential Assay Kit (JC-1).)
Related Product Information for JC-1 assay kit
Principle of the Assay: Mitochondria, the site of most energy production in eukaryotic cells, have a double membrane structure: an outer membrane and a folded inner membrane. Across the inner membrane of intact mitochondria there is a voltage gradient (membrane potential= ??m) with the inside negative and the outside positive. Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. Mitochondrial Membrane Potential Assay Kit (JC JC-1) prov ides a simple method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential, based on a carbocyanine dye, JC JC-1. In healthy cells, this dye accumulates and aggregates in the mitochondria, where it forms bright red fluorescent agglomerates. Any event that dissipates the mitochondrial membrane potential prevents the accumulation of the JC JC-1 dye in the mitochondria and thus, the dye remains in the cytoplasm in its monomer form, leading to a s hift from red (agglomerated JC JC-1, Ex/Em=585/590 nm) to green fluorescence (JC JC-1 monomers, Ex/Em=510/527 nm). JC JC-1 can be used as an indicator of mitochondrial potential in a variety of cell types, including myo myo-cytes and neurons, as well as in intact tissu es and isolated mitochondria. This kit contains CCCP that causes an uncoupling of the proton gradient, which established during the normal activity of elec tron carriers in the electron transport chain, and thus, dissipates the mitochondrial electrochemical potential and may be used as a control that prevents JC JC-1 aggregation.
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Product Notes
The JC-1 (Catalog #AAA330129) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mitochondrial Membrane Potential, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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