Human Klotho ELISA Kit | KL elisa kit
Human Klotho ELISA Kit
Reactivity
Human
Synonyms
Klotho; N/A; Human Klotho ELISA Kit; KL/klotho/KLOTHO; KL elisa kit
Reactivity
Human
Specificity
Specifically recognize KL, no obvious cross reaction with other analogues
Sequence Length
1012
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for KL elisa kit
Background: Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily. Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid.A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti KL antibody was precoated onto the 96-well plate. The biotin conjugated anti KL antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with KL conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of KL in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti KL antibody was precoated onto the 96-well plate. The biotin conjugated anti KL antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with KL conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of KL in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
