Standard Curve (Sample)
(Human Kallikrein 14 PicoKine ELISA Kit standard curve)
Human Kallikrein 14 ELISA Kit | KLK14 elisa kit
Human Kallikrein 14 PicoKine ELISA Kit
Gene Names
KLK14; KLK-L6
Reactivity
Human
Synonyms
Kallikrein 14; N/A; Human Kallikrein 14 PicoKine ELISA Kit; Kallikrein-14; kallikrein-related peptidase 14; hK14; Kallikrein like protein 6; Kallikrein-like protein 6; KLK L6; KLK-L6; KLK14; KLK14_HUMAN; KLK14 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Kallikrein 14
Sequence Length
267
Samples
Cell culture supernates, Cell lysates, serum, plasma (heparin, EDTA) and tissue homogenates
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Kallikrein 14 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Kallikrein 14 PicoKine ELISA Kit standard curve)
Related Product Information for KLK14 elisa kit
Principle of the assay: human Kallikrein 14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 14 has been precoated onto 96-well plates. Standards(NSO, Q19-M248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 14 amount of sample captured in plate.
Background: Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.
Background: Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,122 Da
NCBI Official Full Name
Kallikrein-14
NCBI Official Synonym Full Names
kallikrein-related peptidase 14
NCBI Official Symbol
KLK14
NCBI Official Synonym Symbols
KLK-L6
NCBI Protein Information
kallikrein-14; hK14; kallikrein 14; kallikrein-like protein 6
UniProt Protein Name
Kallikrein-14
UniProt Gene Name
KLK14
UniProt Synonym Gene Names
KLKL6; hK14; KLK-L6
UniProt Entry Name
KLK14_HUMAN
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Product Notes
The Human KLK14 klk14 (Catalog #AAA45750) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45750 ELISA Kit recognizes Human KLK14. It is sometimes possible for the material contained within the vial of "Kallikrein 14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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