Standard Curve (Sample)
(Human Kallikrein 3 PicoKine ELISA Kit standard curve)
Human Kallikrein 3 ELISA Kit | KLK3 elisa kit
Human Kallikrein 3 PicoKine ELISA Kit
Gene Names
KLK3; APS; PSA; hK3; KLK2A1
Reactivity
Human
Synonyms
Kallikrein 3; N/A; Human Kallikrein 3 PicoKine ELISA Kit; Prostate-specific antigen; kallikrein-related peptidase 3; APS; Gamma seminoprotein; Gamma-seminoprotein; hK3; Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; Klk3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Psa; Semenogelase; Seminin; KLK3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Kallikrein 3
Sequence Length
261
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Kallikrein 3 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Kallikrein 3 PicoKine ELISA Kit standard curve)
Related Product Information for KLK3 elisa kit
Principle of the assay: human Kallikrein 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 3 has been precoated onto 96-well plates. Standards(NSO, A18-P261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 3 amount of sample captured in plate.
Background: Prostate-specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome (19q13).
Background: Prostate-specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome (19q13).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,116 Da
NCBI Official Full Name
Prostate-specific antigen
NCBI Official Synonym Full Names
kallikrein-related peptidase 3
NCBI Official Symbol
KLK3
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
NCBI Protein Information
prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
UniProt Protein Name
Prostate-specific antigen
UniProt Gene Name
KLK3
UniProt Synonym Gene Names
APS; PSA; Seminin
UniProt Entry Name
KLK3_HUMAN
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Product Notes
The Human KLK3 klk3 (Catalog #AAA45248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45248 ELISA Kit recognizes Human KLK3. It is sometimes possible for the material contained within the vial of "Kallikrein 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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