Application Data
(GTPase-Glo assay for KRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)
KRAS recombinant protein
Recombinant KRAS (1-186) protein
Synonyms
KRAS; N/A; Recombinant KRAS (1-186) protein; GTPase KRas; KRAS1; KRAS2; Kirsten Rat Sarsoma Viral Oncogene Homolog; C-Ki-Ras; KRAS3B; K-Ras P21 Protein; KRAS recombinant protein
Host
E coli
Form/Format
Recombinant KRAS (1-186) protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant KRAS (1-186) protein that includes amino acids 1-186 of human KRAS protein (accession number NP_203524.1 was expressed in E coli cells with an C-terminal 6xHis tag. The molecular weight of the protein is 22.36kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(GTPase-Glo assay for KRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)
Application Data
(GTPase-Glo assay for KRAS (1-186) activity 1uM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5ul. To the completed GTPase reactions, we added 5ul of reconstituted GTPase-Glo Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10ul) was added, reactions were incubated for 10 minutes and luminescence was measured.)
SDS-PAGE
(Recombinant KRAS (1-186) protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 22.36kDa Purity: >90%)
SDS-PAGE
(Recombinant KRAS (1-186) protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 22.36kDa Purity: >90%)
Related Product Information for KRAS recombinant protein
KRAS (GTPase KRas) protein is a GDP/GTP binding protein, and the more important genes of the same family include HRAS and NRAS. KRAS is activated when combined with GTP and closed when combined with GDP. KRAS can be transiently activated by growth factors or tyrosine kinases (such as EGFR). The activated KRAS can activate downstream signals such as PI3K-AKT-mTOR that control cell production pathway, and the RAS-RAF-MEK-ERK signaling pathway that controls cell proliferation. Mutant KRAS will continue to be activated even without activation of kinases such as EGFR, resulting in continued cell proliferation and eventually canceration. KRAS mutations are found in a variety of tumors, the most common ones include lung cancer and pancreatic cancer.
Product Categories/Family for KRAS recombinant protein
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Product Notes
The KRAS (Catalog #AAA60374) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KRAS, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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