Keratin, type I cytoskeletal 10 Recombinant Protein | KRT10 recombinant protein

Recombinant Human Keratin, type I cytoskeletal 10

Cat. Num. AAA81713

• Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Keratin, type I cytoskeletal 10; N/A; Recombinant Human Keratin, type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10; KRT10 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-584a; Full Length
• Sequence: MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Product Categories/Family for KRT10 recombinant protein

Signal Transduction

References

The complete sequence of the human intermediate filament chain keratin 10. Subdomainal divisions and model for folding of end domain sequences.Zhou X.M., Idler W.W., Steven A.C., Roop D.R., Steinert P.M.J. Biol. Chem. 263:15584-15589(1988) Identification of an orthologous mammalian cytokeratin gene. High degree of intron sequence conservation during evolution of human cytokeratin 10.Rieger M., Franke W.W.J. Mol. Biol. 204:841-856(1988) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.Nature 440:1045-1049(2006) Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their tissue-specific expression.Darmon M.Y., Semat A., Darmon M.C., Vasseur M.Mol. Biol. Rep. 12:277-283(1987) Prenatal diagnosis of epidermolytic hyperkeratosis by direct gene sequencing.Rothnagel J.A., Longley M.A., Holder R.A., Kuster W., Roop D.R.J. Invest. Dermatol. 102:13-16(1994) Identification of mutational hot spots in the suprabasal keratin genes from patients with epidermolytic hyperkeratosis.Rothnagel J.J., Dominey A., Fisher M., Axtell S., Pittelkow M., Anton-Lamprecht I., Hohl D., Roop D.Exons I and VII of the gene (Ker10) encoding human keratin 10 undergo structural rearrangements within repeats.Tkachenko A.V., Buchman V.L., Bliskovsky V.V., Shvets Y.P., Kisselev L.L.Gene 116:245-251(1992) Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.Electrophoresis 13:960-969(1992) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.Mol. Cell 31:438-448(2008) Mitotic recombination in patients with ichthyosis causes reversion of dominant mutations in KRT10.Choate K.A., Lu Y., Zhou J., Choi M., Elias P.M., Farhi A., Nelson-Williams C., Crumrine D., Williams M.L., Nopper A.J., Bree A., Milstone L.M., Lifton R.P.Science 330:94-97(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) The genetic basis of epidermolytic hyperkeratosis a disorder of differentiation-specific epidermal keratin genes.Cheng J., Syder A.J., Yu Q.-C., Letai A., Paller A.S., Fuchs E.Cell 70:811-819(1992) Extensive size polymorphism of the human keratin 10 chain resides in the C-terminal V2 subdomain due to variable numbers and sizes of glycine loops.Korge B.P., Gan S.-Q., McBridge O.W., Mischke D., Steinert P.M.Proc. Natl. Acad. Sci. U.S.A. 89:910-914(1992) Mutations in the rod domains of keratins 1 and 10 in epidermolytic hyperkeratosis.Rothnagel J.A., Dominey A.M., Dempsey L.D., Longley M.A., Greenhalgh D.A., Gagne T.A., Huber M., Frenk E., Hohl D., Roop D.R.Science 257:1128-1130(1992) Preferential sites in keratin 10 that are mutated in epidermolytic hyperkeratosis.Chipev C.C., Yang J.-M., Digiovanna J.J., Steinert P.M., Marekov L., Compton J.G., Bale S.J.Am. J. Hum. Genet. 54:179-190(1994) Genetic mutations in the K1 and K10 genes of patients with epidermolytic hyperkeratosis. Correlation between location and disease severity.Syder A.J., Yu Q.-C., Paller A.S., Giudice G., Pearson R., Fuchs E.J. Clin. Invest. 93:1533-1542(1994) Mutations in the rod 1A domain of keratins 1 and 10 in bullous congenital ichthyosiform erythroderma (BCIE) .McLean W.H.I., Eady R.A.J., Dopping-Hepenstal P.J.C., McMillan J.R., Leigh I.M., Navsaria H.A., Higgins C., Harper J.I., Paige D.G., Morley S.M.J. Invest. Dermatol. 102:24-30(1994) Genetic and clinical mosaicism in a type of epidermal nevus.Paller A.S., Syder A.J., Chan Y.-M., Yu Q.-C., Hutton M.E., Tadini G., Fuchs E.N. Engl. J. Med. 331:1408-1415(1994) A novel dinucleotide mutation in keratin 10 in the annular epidermolytic ichthyosis variant of bullous congenital ichthyosiform erythroderma.Joh G.-Y., Traupe H., Metze D., Nashan D., Huber M., Hohl D., Longley M.A., Rothnagel J.A., Roop D.R.J. Invest. Dermatol. 108:357-361(1997) A novel helix termination mutation in keratin 10 in annular epidermolytic ichthyosis, a variant of bullous congenital ichthyosiform erythroderma.Suga Y., Duncan K.O., Heald P.W., Roop D.R.J. Invest. Dermatol. 111:1220-1223(1998) A novel substitution in keratin 10 in epidermolytic hyperkeratosis.Arin M.J., Longley M.A., Anton-Lamprecht I., Kurze G., Huber M., Hohl D., Rothnagel J.A., Roop D.R.J. Invest. Dermatol. 112:506-508(1999) Expanding the keratin mutation database novel and recurrent mutations and genotype-phenotype correlations in 28 patients with epidermolytic ichthyosis.Arin M.J., Oji V., Emmert S., Hausser I., Traupe H., Krieg T., Grimberg G.Br. J. Dermatol. 164:442-447(2011)

NCBI and Uniprot Product Information

• NCBI GI #: 195972866
• NCBI GeneID: 3858
• NCBI Accession #: NP_000412.3
• NCBI GenBank Nucleotide #: NM_000421.3
• UniProt Accession #: P13645; Q14664; Q8N175
• Molecular Weight: 62.8 kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10, type I
• NCBI Official Symbol: KRT10
• NCBI Official Synonym Symbols: BIE; EHK; K10; KPP; BCIE; CK10
• NCBI Protein Information: keratin, type I cytoskeletal 10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• UniProt Gene Name: KRT10
• UniProt Synonym Gene Names: KPP; CK-10; K10
• UniProt Entry Name: K1C10_HUMAN