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product-image-AAA133972_SDS_PAGE15.jpg SDS-PAGE

Keratin 71 (KRT71) Recombinant Protein | KRT71 recombinant protein

Recombinant Keratin 71 (KRT71)

Gene Names
Krt71; Ca; Cu; Cal4; mK6irs; Krt2-6g; mK6irs1; AA589543
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Keratin 71 (KRT71); N/A; Recombinant Keratin 71 (KRT71); KRT71 recombinant protein
Ordering
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-EREQIKALNN KFASFIDKVR FLEQQNQVLQ TKWELLQQLD LNNCKNNLEP ILEGHISNMR KQLETLSGDR VRLDSELRNV RDVVEDYKKK YEEEINRRTA AENEFVLLKK DVDAAYANKV ELQAKVDTMD QDIKFFKCLF EAEMAQIQSH ISDMSVILSM DNNRNLDLDS IIDEVRAQYE EIALKSKAEA EALYQTKFQE LQLAAGRHGD DLKNTKNEIT ELTRFIQRLR SEIENAKKQA SNLETAIADA EQRGDSALKD ARAKLDELEG ALHQAKEELA RMLREYQELM SLKLALDMEI ATYRKLLESE
Sequence Length
524
Applicable Applications for KRT71 recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Glu131~Glu440 (Accession # Q9R0H5) with N-terminal His-Tag
Predicted isoelectric point
5.4
Predicted Molecular Mass
37.2kDa
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE

product-image-AAA133972_SDS_PAGE15.jpg SDS-PAGE

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type II cytoskeletal 71
NCBI Official Synonym Full Names
keratin 71
NCBI Official Symbol
Krt71
NCBI Official Synonym Symbols
Ca; Cu; Cal4; mK6irs; Krt2-6g; mK6irs1; AA589543
NCBI Protein Information
keratin, type II cytoskeletal 71; K6G; K71; CK-6G; CK-71; caracul; keratin-6G; keratin-71; cytokeratin-6G; cytokeratin-71; mK6irs1/Krt2-6g; type-II keratin Kb34; keratin complex 2, gene 6g; keratin complex 2, basic, gene 6g; type II inner root sheath-specific keratin-K6irs1
UniProt Protein Name
Keratin, type II cytoskeletal 71
UniProt Gene Name
Krt71
UniProt Synonym Gene Names
K6irs1; Kb34; Krt2-6g; Krt6g; CK-6G; CK-71; K6G; K71; mK6irs
UniProt Entry Name
K2C71_MOUSE

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Product Notes

The KRT71 krt71 (Catalog #AAA133972) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Keratin 71 (KRT71) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the KRT71 krt71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-EREQIK ALNN KFASFIDKVR FLEQQNQVLQ TKWELLQQLD LNNCKNNLEP ILEGHISNMR KQLETLSGDR VRLDSELRNV RDVVEDYKKK YEEEINRRTA AENEFVLLKK DVDAAYANKV ELQAKVDTMD QDIKFFKCLF EAEMAQIQSH ISDMSVILSM DNNRNLDLDS IIDEVRAQYE EIALKSKAEA EALYQTKFQE LQLAAGRHGD DLKNTKNEIT ELTRFIQRLR SEIENAKKQA SNLETAIADA EQRGDSALKD ARAKLDELEG ALHQAKEELA RMLREYQELM SLKLALDMEI ATYRKLLESE. It is sometimes possible for the material contained within the vial of "Keratin 71 (KRT71), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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