Standard Curve (Sample)
Human Laminin ELISA Kit | LAMA1 elisa kit
Human Laminin PicoKine ELISA Kit
Gene Names
LAMA3; E170; LOCS; BM600; LAMNA; lama3a
Reactivity
Human
Synonyms
Laminin; N/A; Human Laminin PicoKine ELISA Kit; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S); Lamb2; LAMB2_HUMAN; LAMC1; LAMC2; LAMC3; laminin A; Laminin B; Laminin B1s chain; Laminin gamma 1; Laminin gamma 2; Laminin gamma 3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; OCCM; S-LAM beta; S-laminin subunit beta; LAMA1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human LAMA1; LAMB1; LAMC1
Sequence Length
1,668
Samples
Cell culture supernatants, cell lysates and serum
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LAMA1 elisa kit
Background: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
Principle of the Assay: The Picokine™ Human LAMA1; LAMB1; LAMC1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LAMA1; LAMB1; LAMC1 with a 96-well strip plate that is pre-coated with antibody specific for LAMA1; LAMB1; LAMC1. The detection antibody is a biotinylated antibody specific for LAMA1; LAMB1; LAMC1. The capture antibody is polyclonal antibody from rabbit and the detection antibody is polyclonal antibody from rabbit. The kit contains recombinant Human LAMA1; LAMB1; LAMC1 with immunogen: Expression system for standard: from human fibroblasts. The kit is analytically validated with ready-to-use reagents. To measure Human LAMA1; LAMB1; LAMC1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LAMA1; LAMB1; LAMC1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LAMA1; LAMB1; LAMC1 in the sample.
Principle of the Assay: The Picokine™ Human LAMA1; LAMB1; LAMC1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LAMA1; LAMB1; LAMC1 with a 96-well strip plate that is pre-coated with antibody specific for LAMA1; LAMB1; LAMC1. The detection antibody is a biotinylated antibody specific for LAMA1; LAMB1; LAMC1. The capture antibody is polyclonal antibody from rabbit and the detection antibody is polyclonal antibody from rabbit. The kit contains recombinant Human LAMA1; LAMB1; LAMC1 with immunogen: Expression system for standard: from human fibroblasts. The kit is analytically validated with ready-to-use reagents. To measure Human LAMA1; LAMB1; LAMC1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LAMA1; LAMB1; LAMC1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LAMA1; LAMB1; LAMC1 in the sample.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
184,054 Da
NCBI Official Synonym Full Names
laminin, alpha 3
NCBI Official Symbol
LAMA3
NCBI Official Synonym Symbols
E170; LOCS; BM600; LAMNA; lama3a
NCBI Protein Information
laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)
UniProt Protein Name
Laminin subunit alpha-3
UniProt Gene Name
LAMA3
UniProt Synonym Gene Names
LAMNA; E170
UniProt Entry Name
LAMA3_HUMAN
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Product Notes
The Human LAMA1 lama3 (Catalog #AAA45321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45321 ELISA Kit recognizes Human LAMA1. It is sometimes possible for the material contained within the vial of "Laminin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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