Rat lipopolysaccharide binding protein ELISA Kit | LBP elisa kit
Rat lipolysaccharide binding protein, LBP ELISA Kit
Reactivity
Rat
Synonyms
lipopolysaccharide binding protein; N/A; Rat lipolysaccharide binding protein, LBP ELISA Kit; Rat lipolysaccharide binding protein; LBP ELISA Kit; MGC22233; LPS-binding protein; lipopolysaccharide-binding protein; LBP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat LBP. No significant cross-reactivity or interference between rat LBP and analogues was observed.
Sequence Length
482
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for LBP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
