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product-image-AAA48386_SDS_PAGE15.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

LBP recombinant protein

Recombinant human LBP protein

Gene Names
LBP; BPIFD2
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
LBP; N/A; Recombinant human LBP protein; Lipopolysaccharide-binding protein; BPIFD2; MGC22233; LCD-binding protein; OTTHUMP00000030965; LBP recombinant protein
Ordering
Host
HEK293
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
Sequence Positions
26-481aa
Sequence
ANPGLVARIT DKGLQYAAQE GLLALQSELL RITLPDFTGD LRIPHVGRGR YEFHSLNIHS CELLHSALRP VPGQGLSLSI SDSSIRVQGR WKVRKSFFKL QGSFDVSVKG ISISVNLLLG SESSGRPTVT ASSCSSDIAD VEVDMSGDLG WLLNLFHNQI ESKFQKVLES RICEMIQKSV SSDLQPYLQT LPVTTEIDSF ADIDYSLVEA PRATAQMLEV MFKGEIFHRN HRSPVTLLAA VMSLPEEHNK MVYFAISDYV FNTASLVYHE EGYLNFSITD DMIPPDSNIR LTTKSFRPFV PRLARLYPNM NLELQGSVPS APLLNFSPGN LSVDPYMEID AFVLLPSSSK EPVFRLSVAT NVSATLTFNT SKITGFLKPG KVKVELKESK VGLFNAELLE ALLNYYILNT FYPKFNDKLA EGFPLPLLKR VQLYDLGLQI HKDFLFLGAN VQYMRV
Applicable Applications for LBP recombinant protein
SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-term
Ala 26
Preparation and Storage
For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

product-image-AAA48386_SDS_PAGE15.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for LBP recombinant protein
LBP, also known as Lipopolysaccharide-binding protein, is a glycoprotein member of the BPI/LBP family of lipid-binding proteins. LBP is a glycoprotein that is synthesized principally by hepatocytes. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. It binds to LPS to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. Recombinant human LBP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for LBP recombinant protein
References
J. Weiss, 2003, Biochemical Society Transactions: Volume 31, part 4: 785-790. Hubacek J.A., et al., 1997, Biochem. Biophys. Res. Commun. 236:427-430.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51.7kDa (462aa)
NCBI Official Full Name
lipopolysaccharide-binding protein
NCBI Official Synonym Full Names
lipopolysaccharide binding protein
NCBI Official Symbol
LBP
NCBI Official Synonym Symbols
BPIFD2
NCBI Protein Information
lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2
UniProt Protein Name
Lipopolysaccharide-binding protein
UniProt Gene Name
LBP
UniProt Synonym Gene Names
LBP
UniProt Entry Name
LBP_HUMAN

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Product Notes

The LBP lbp (Catalog #AAA48386) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 26-481aa. AAA Biotech's LBP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the LBP lbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ANPGLVARIT DKGLQYAAQE GLLALQSELL RITLPDFTGD LRIPHVGRGR YEFHSLNIHS CELLHSALRP VPGQGLSLSI SDSSIRVQGR WKVRKSFFKL QGSFDVSVKG ISISVNLLLG SESSGRPTVT ASSCSSDIAD VEVDMSGDLG WLLNLFHNQI ESKFQKVLES RICEMIQKSV SSDLQPYLQT LPVTTEIDSF ADIDYSLVEA PRATAQMLEV MFKGEIFHRN HRSPVTLLAA VMSLPEEHNK MVYFAISDYV FNTASLVYHE EGYLNFSITD DMIPPDSNIR LTTKSFRPFV PRLARLYPNM NLELQGSVPS APLLNFSPGN LSVDPYMEID AFVLLPSSSK EPVFRLSVAT NVSATLTFNT SKITGFLKPG KVKVELKESK VGLFNAELLE ALLNYYILNT FYPKFNDKLA EGFPLPLLKR VQLYDLGLQI HKDFLFLGAN VQYMRV. It is sometimes possible for the material contained within the vial of "LBP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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