LCN1 recombinant protein

LCN1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257979

• Gene Names: LCN1; TP; TLC; PMFA; VEGP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LCN1; N/A; LCN1 Protein, Human, Recombinant (His Tag); Human LCN1/VEGP/Lipocalin-1 Protein (His Tag); Lipocalin 1 Protein, Human; MGC71975 Protein, Human; PMFA Protein, Human; TLC Protein, Human; TP Protein, Human; VEGP Protein, Human; lipocalin 1; LCN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met1-Asp176

• Source Note: HEK293 Cells-derived Human LCN1 / VEGP / Lipocalin-1 Protein (His Tag); Met1-Asp176, Accession # NP_002288.1
• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LCN1 (NP_002288.1) extracellular domain (Met 1-Asp176) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: Reconstituted with sterile deionized water to 0.25 mg/mL
• Molecular Mass Note: The secreted recombinant human LCN1 consists of 169 amino acids and has a predicted molecular mass of 19 kDa The apparent molecular mass of the protein is approximately 20 kDa in SDS-PAGE under reducing conditions.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-PAGE

Related Product Information for LCN1 recombinant protein

Background: Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear Prealbumin, VEGP, Tear lipocalin, and LCN1 is a secreted protein that belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1/VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1/VEGP is identical to lingual von Ebner's gland protein and is also produced in the prostate, nasal mucosa, and tracheal mucosa. Homologous proteins have been found in the rat, pig, and probably dog and horse. Lipocalin-1/VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1/VEGP acts as the principal lipid-binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1/VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1/LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1/LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.

References

Lassagne H. et al., 1993, Exp. Eye Res. 56:605-609. Redl,B. et al., 2000, Biochim Biophys Acta 1482 (1-2):241-8. Wojnar P. et al., 2001, J. Biol. Chem. 276:20206-20212. Wojnar P. et al., 2003, J. Biol. Chem. 278:16209-16215. Breustedt D.A. et al., 2005, J. Biol. Chem. 280:484-493.

NCBI and Uniprot Product Information

• NCBI GI #: 4504963
• NCBI GeneID: 3933
• NCBI Accession #: NP_002288.1
• NCBI GenBank Nucleotide #: NM_002297.3
• UniProt Accession #: P31025; Q5T8A1
• Molecular Weight: Predicted Molecular Mass 18.89 kDa
• NCBI Official Full Name: lipocalin-1 isoform 1
• NCBI Official Synonym Full Names: lipocalin 1
• NCBI Official Symbol: LCN1
• NCBI Official Synonym Symbols: TP; TLC; PMFA; VEGP
• NCBI Protein Information: lipocalin-1; VEG protein; tear prealbumin; Von Ebner gland protein; lipocalin 1 (tear prealbumin); protein migrating faster than albumin
• UniProt Protein Name: Lipocalin-1
• UniProt Gene Name: LCN1
• UniProt Synonym Gene Names: VEGP; Tlc; TP; VEG protein
• UniProt Entry Name: LCN1_HUMAN