Standard Curve (Sample)
Human Lipocalin-2/NGAL ELISA Kit | LCN2 elisa kit
Human Lipocalin-2/NGAL PicoKine ELISA Kit
Gene Names
LCN2; 24p3; MSFI; NGAL
Reactivity
Human
Synonyms
Lipocalin-2/NGAL; N/A; Human Lipocalin-2/NGAL PicoKine ELISA Kit; Neutrophil gelatinase-associated lipocalin; lipocalin 2; 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; Lcn2; Lipocalin 2; Lipocalin-2; Migration stimulating factor inhibitor; MSFI; NGAL; NGAL_HUMAN; Oncogene 24p3; p25; Siderocalin; LCN2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human LCN2
Sequence Length
198
Samples
Cell culture supernatants, serum, plasma (heparin), saliva and urine
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LCN2 elisa kit
Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.
Principle of the Assay: The Picokine™ Human LCN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LCN2 with a 96-well strip plate that is pre-coated with antibody specific for LCN2. The detection antibody is a biotinylated antibody specific for LCN2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LCN2 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-G198. The kit is analytically validated with ready-to-use reagents. To measure Human LCN2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LCN2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LCN2 in the sample.
Principle of the Assay: The Picokine™ Human LCN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LCN2 with a 96-well strip plate that is pre-coated with antibody specific for LCN2. The detection antibody is a biotinylated antibody specific for LCN2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LCN2 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-G198. The kit is analytically validated with ready-to-use reagents. To measure Human LCN2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LCN2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LCN2 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,457 Da
NCBI Official Full Name
Neutrophil gelatinase-associated lipocalin
NCBI Official Synonym Full Names
lipocalin 2
NCBI Official Symbol
LCN2
NCBI Official Synonym Symbols
24p3; MSFI; NGAL
NCBI Protein Information
neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
LCN2
UniProt Synonym Gene Names
HNL; NGAL; NGAL
UniProt Entry Name
NGAL_HUMAN
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Product Notes
The Human LCN2 lcn2 (Catalog #AAA11538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11538 ELISA Kit recognizes Human LCN2. It is sometimes possible for the material contained within the vial of "Lipocalin-2/NGAL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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