Application Data
Human Lipocalin-2/NGAL ELISA Kit | LCN2 elisa kit
Human Lipocalin-2/NGAL Quick ELISA Kit
Reactivity
Human
Synonyms
Lipocalin-2/NGAL; N/A; Human Lipocalin-2/NGAL Quick ELISA Kit; LCN2; HNL; NGAL; Neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin; p25; lipocalin 2; LCN2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human LCN2
Assay Type
Colorimetric sandwich
Samples
cell culture supernatants, serum, plasma (heparin), saliva and urine
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Capture/Detection Antibodies
Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
Sample Data
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000
O.D.|0.030|0.194|0.330|0.588|1.035|1.606|2.212|2.599
O.D.|0.030|0.194|0.330|0.588|1.035|1.606|2.212|2.599
Intra-Assay Precision
Sample|1|2|3|
n|16|16|16|
Mean(pg/ml)|285|1624|5541|
Standard deviation|18.52|86.07|243.8|
CV(%)|6.5%|5.3%|4.4%|
n|16|16|16|
Mean(pg/ml)|285|1624|5541|
Standard deviation|18.52|86.07|243.8|
CV(%)|6.5%|5.3%|4.4%|
Inter-Assay Precision
Sample|1|2|3|
n|24|24|24
Mean(pg/ml)|290|1737|5384
Standard deviation|22.04|99|285.35
CV(%)|7.6%|5.7%|5.3%
n|24|24|24
Mean(pg/ml)|290|1737|5384
Standard deviation|22.04|99|285.35
CV(%)|7.6%|5.7%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 285|255|236|267|253|15.43|6.1%
Sample 2: 1624|1649|1587|1810|1667|120.02|7.2%
Sample 3: 5541|5497|5323|4757|5279|306.18|5.8%
*number of samples for each test n=16.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 285|255|236|267|253|15.43|6.1%
Sample 2: 1624|1649|1587|1810|1667|120.02|7.2%
Sample 3: 5541|5497|5323|4757|5279|306.18|5.8%
*number of samples for each test n=16.
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-G198
Anticoagulant
heparin
Benefits
The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human LCN2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Principle
Human LCN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human LCN2 in cell culture supernatants, serum, plasma (heparin), saliva and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human LCN2 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-G198.
To measure Human LCN2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human LCN2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LCN2 in the sample.
To measure Human LCN2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human LCN2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LCN2 in the sample.
Assay Procedure Step 1
10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
TMB Incubation Time
15
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)
Application Data
Application Data
Related Product Information for LCN2 elisa kit
Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. 1 The LCN2 gene is located at 9q34 and contains 7 exons. 2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. 3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. 4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.
Product Categories/Family for LCN2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
22,588 Da
NCBI Official Full Name
LCN2
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
LCN2
UniProt Synonym Gene Names
HNL; NGAL; NGAL
UniProt Entry Name
NGAL_HUMAN
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Product Notes
The Human LCN2 lcn2 (Catalog #AAA125877) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125877 ELISA Kit recognizes Human LCN2. It is sometimes possible for the material contained within the vial of "Lipocalin-2/NGAL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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