Standard Curve (Sample)
Human NGAL ELISA Kit | LCN2 elisa kit
Human NGAL ELISA Kit
Gene Names
LCN2; 24p3; MSFI; NGAL
Reactivity
Human
Applications
ELISA
Synonyms
NGAL; N/A; Human NGAL ELISA Kit; HNL; Neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25; LCN2 elisa kit
Reactivity
Human
Specificity
The Human NGAL ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human NGAL proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
198
Samples
Culture Supernates, Serum, Plasma, Tissue Homogenates, Saliva, Urine
Sensitivity
< 40 pg/ml
Applicable Applications for LCN2 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LCN2 elisa kit
Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. The LCN2 gene is located at 9q34 and contains 7 exons. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.
Principle of the Assay: The AAA Biotech' Human NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NGAL in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human NGAL coated on a 96-well plate. Standards and samples are pipetted into the wells and NGAL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGAL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGAL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The AAA Biotech' Human NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NGAL in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human NGAL coated on a 96-well plate. Standards and samples are pipetted into the wells and NGAL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGAL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGAL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,457 Da
NCBI Official Full Name
neutrophil gelatinase-associated lipocalin
NCBI Official Synonym Full Names
lipocalin 2
NCBI Official Symbol
LCN2
NCBI Official Synonym Symbols
24p3; MSFI; NGAL
NCBI Protein Information
neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
LCN2
UniProt Synonym Gene Names
HNL; NGAL; NGAL
UniProt Entry Name
NGAL_HUMAN
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Product Notes
The Human LCN2 lcn2 (Catalog #AAA105520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA105520 ELISA Kit recognizes Human LCN2. AAA Biotech's NGAL can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the LCN2 lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGAL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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