Standard Curve (Sample)
(Mouse Lipocalin-2/NGAL PicoKine ELISA Kit standard curve)
Mouse Lipocalin-2/NGAL ELISA Kit | LCN2 elisa kit
Mouse Lipocalin-2/NGAL PicoKine ELISA Kit
Gene Names
Lcn2; 24p3; Sip24; AW212229
Reactivity
Mouse
Synonyms
Lipocalin-2/NGAL; N/A; Mouse Lipocalin-2/NGAL PicoKine ELISA Kit; Neutrophil gelatinase-associated lipocalin; lipocalin 2; 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; Lcn2; Lipocalin 2; Lipocalin-2; Migration stimulating factor inhibitor; MSFI; NGAL; NGAL_HUMAN; Oncogene 24p3; p25; Siderocalin; LCN2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse NGAL
Sequence Length
200
Samples
Cell culture supernates, serum, plasma (heparin) and urine
Assay Type
Sandwich
Detection Range
78pg/ml-5,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse Lipocalin-2/NGAL PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse Lipocalin-2/NGAL PicoKine ELISA Kit standard curve)
Related Product Information for LCN2 elisa kit
Principle of the assay: mouse NGAL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGAL has been precoated onto 96-well plates. Standards(NSO, Q21-N200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGAL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGAL amount of sample captured in plate.
Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene islocated at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances suchas bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adultbone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.
Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene islocated at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances suchas bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adultbone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,875 Da
NCBI Official Full Name
Neutrophil gelatinase-associated lipocalin
NCBI Official Synonym Full Names
lipocalin 2
NCBI Official Symbol
Lcn2
NCBI Official Synonym Symbols
24p3; Sip24; AW212229
NCBI Protein Information
neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; siderocalin LCN2; SV-40-induced 24P3 protein; secreted inducible protein 24
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
Lcn2
UniProt Synonym Gene Names
NGAL
UniProt Entry Name
NGAL_MOUSE
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Product Notes
The Mouse LCN2 lcn2 (Catalog #AAA45361) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45361 ELISA Kit recognizes Mouse LCN2. It is sometimes possible for the material contained within the vial of "Lipocalin-2/NGAL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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