Human lactate dehydrogenase D ELISA Kit | LDHD elisa kit

Human D-Lactate Dehydrogenase, D-LDH ELISA Kit

Cat. Num. AAA79662

• Gene Names: LDHD; DLD
• Reactivity: Human
• Synonyms: lactate dehydrogenase D; N/A; Human D-Lactate Dehydrogenase, D-LDH ELISA Kit; Human D-Lactate Dehydrogenase; D-LDH ELISA Kit; MGC57726; D-lactate dehydrogenase; LDHD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human LDHD. No significant cross-reactivity or interference between Human LDHD and analogues was observed.
• Sequence Length: 507
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 31.25 mIU/ml-2000 mIU/ml
• Sensitivity: 7.8 mIU/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for LDHD elisa kit

Introduction: Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. LDH is found in the liver, kidneys, striated muscle, and heart muscle that catalyze the reversible conversion of pyruvate and lactate. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis to lactic acid when oxygen is absent or in short supply, and it performs the reverse reaction during the cori cycle in the liver. At high concentrations of lactate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. Tissue breakdown elevates levels of LDH, and therefore a measure of it indicates e.g. hemolysis. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis and HIV. Two forms of lactate dehydrogenase with different substrate specificities have been found; L-lactate dehydrogenase and D-lactate dehydrogenase. Whereas the enzyme from vertebrates and plants only forms L-lactate, invertebrates, lower fungi, and prokaryotic organisms possess either a D- or L-lactate specific dehydrogenase, depending on the species. Some prokaryotes even possess both enzymes within the same cell. On the other hand, eukaryotes have several isozyme forms, but all have a single stereospecificity.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well 4 and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37595754
• NCBI GeneID: 197257
• NCBI Accession #: NP_705690.2
• NCBI GenBank Nucleotide #: NM_153486.3
• UniProt Accession #: Q86WU2; Q8IZK5
• Molecular Weight: 54,871 Da
• NCBI Official Full Name: probable D-lactate dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: lactate dehydrogenase D
• NCBI Official Symbol: LDHD
• NCBI Official Synonym Symbols: DLD
• NCBI Protein Information: probable D-lactate dehydrogenase, mitochondrial
• UniProt Protein Name: Probable D-lactate dehydrogenase, mitochondrial
• UniProt Gene Name: LDHD
• UniProt Synonym Gene Names: DLD; Lactate dehydrogenase D
• UniProt Entry Name: LDHD_HUMAN