Standard Curve (Sample)
(Fig.1. Human Leukocyte elastase inhibitor (SERPINB1) Standard Curve.)
Human Leukocyte Elastase Inhibitor (SERPINB1) ELISA Kit | LEI elisa kit
Human Leukocyte Elastase Inhibitor (SERPINB1) ELISA Kit
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
Reactivity
Human
Synonyms
Leukocyte Elastase Inhibitor (SERPINB1); N/A; Human Leukocyte Elastase Inhibitor (SERPINB1) ELISA Kit; SERPINB1; EI; ELANH2; M/NEI; MNEI; PI2; anti-elastase; protease inhibitor 2 (anti-elastase); monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 1; LEI elisa kit
Reactivity
Human
Specificity
Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINB1. No significant cross-reactivity or interference between Human SERPINB1 and analogues was observed.
Sequence Length
379
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Leukocyte elastase inhibitor (SERPINB1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Leukocyte elastase inhibitor (SERPINB1) Standard Curve.)
Related Product Information for LEI elisa kit
Description: This Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB1 in samples. An antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI).
Background: Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin family B member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
NCBI Protein Information
leukocyte elastase inhibitor
UniProt Protein Name
Leukocyte elastase inhibitor
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
UniProt Entry Name
ILEU_HUMAN
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Product Notes
The Human LEI serpinb1 (Catalog #AAA330909) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330909 ELISA Kit recognizes Human LEI. It is sometimes possible for the material contained within the vial of "Leukocyte Elastase Inhibitor (SERPINB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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