Mouse Lactoferrin ELISA Kit | LF elisa kit

Mouse Lactoferrin ELISA Kit

Cat. Num. AAA16263

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Mouse
• Synonyms: Lactoferrin; N/A; Mouse Lactoferrin ELISA Kit; LF elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LTF. No significant cross-reactivity or interference between LTF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTF and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ?g/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for LF elisa kit

Principle of the Assay: LTF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LTF antibody and an LTF-HRP conjugate. The assay sample and buffer are incubated together with LTF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTF concentration since LTF from samples and LTF-HRP conjugate compete for the anti-LTF antibody binding site. Since the number of sites is limited, as more sites are occupied by LTF from the sample, fewer sites are left to bind LTF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTF concentration in each sample is interpolated from this standard curve.

Intended Uses: This LTF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LTF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for LF elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 187122
• NCBI GeneID: 4057
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactoferrin
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110
• UniProt Protein Name: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN