Standard Curve (Sample)
(Human Galectin-4 PicoKine ELISA Kit standard curve)
Human Galectin-4 ELISA Kit | LGALS4 elisa kit
Human Galectin-4 PicoKine ELISA Kit
Gene Names
LGALS4; GAL4; L36LBP
Reactivity
Human
Synonyms
Galectin-4; N/A; Human Galectin-4 PicoKine ELISA Kit; lectin, galactoside-binding, soluble, 4; Antigen NY-CO-27; Gal-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; LEG4_HUMAN; LGALS4; LGALS4 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Galectin-4
Sequence Length
323
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Galectin-4 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Galectin-4 PicoKine ELISA Kit standard curve)
Related Product Information for LGALS4 elisa kit
Principle of the assay: human Galectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been precoated onto 96-well plates. Standards(E.coli, A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-4 amount of sample captured in plate.
Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,941 Da
NCBI Official Full Name
Galectin-4
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 4
NCBI Official Symbol
LGALS4
NCBI Official Synonym Symbols
GAL4; L36LBP
NCBI Protein Information
galectin-4; gal-4; galectin 4; antigen NY-CO-27; lactose-binding lectin 4; L-36 lactose-binding protein
UniProt Protein Name
Galectin-4
UniProt Gene Name
LGALS4
UniProt Synonym Gene Names
Gal-4; L36LBP
UniProt Entry Name
LEG4_HUMAN
Similar Products
Product Notes
The Human LGALS4 lgals4 (Catalog #AAA45819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45819 ELISA Kit recognizes Human LGALS4. It is sometimes possible for the material contained within the vial of "Galectin-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.