LIF recombinant protein

LIF Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257446

• Gene Names: LIF; CDF; DIA; HILDA; MLPLI
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: LIF; N/A; LIF Protein, Human, Recombinant (His Tag); Human LIF Protein (His Tag); leukemia inhibitory factor; CDF Protein, Human; DIA Protein, Human; HILDA Protein, Human; Leukemia Inhibitory Factor Protein, Human; MLPLI Protein, Human; LIF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 191aa
• Sequence: Met1-Phe202

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser23
• Protein Construction: |A DNA sequence encoding the human LIF (P15018) (Met1-Phe202) with a C-terminal polyhistidine tag was expressed
• Activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 2-15ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 2-15ng/mL.)

Application Data

Related Product Information for LIF recombinant protein

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-alpha) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.

References

Salas EM, et al. (2011) LIF, a Novel STAT5-Regulated Gene, Is Aberrantly Expressed in Myeloproliferative Neoplasms. Genes Cancer. 2 (5): 593-6.Chodorowska G, et al. (2004) Leukemia inhibitory factor (LIF) and its biological activity. Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93.Garcia-Campana AM, et al. (2007) LIF detection of peptides and proteins in CE. Electrophoresis. 28 (1-2): 208-32.

NCBI and Uniprot Product Information

• NCBI GI #: 4504991
• NCBI GeneID: 3976
• NCBI Accession #: NP_002300.1
• NCBI GenBank Nucleotide #: NM_002309.4
• UniProt Accession #: P15018; Q52LZ2; B2RCW7; B5MC23
• Molecular Weight: 22,008 Da
• NCBI Official Full Name: leukemia inhibitory factor isoform 1
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: LIF
• NCBI Official Synonym Symbols: CDF; DIA; HILDA; MLPLI
• NCBI Protein Information: leukemia inhibitory factor; D factor; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation-stimulating factor; differentiatio
• UniProt Protein Name: Leukemia inhibitory factor
• UniProt Gene Name: LIF
• UniProt Synonym Gene Names: HILDA; LIF; D factor; MLPLI
• UniProt Entry Name: LIF_HUMAN