Listeriolysin-O Active Protein | LLO active protein
Recombinant Listeriolysin-O
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Listeriolysin-O; N/A; Recombinant Listeriolysin-O; LLO; hlyA; LLO active protein
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered clear solution
The protein (0.75 mg/mL) contains 50mM NaH2PO4, 1mM EDTA, 2.7 mM KCl, pH 6.4, 1 mM DTT, 5% (v/v) glycerol and 0.5M NaCl.
The protein (0.75 mg/mL) contains 50mM NaH2PO4, 1mM EDTA, 2.7 mM KCl, pH 6.4, 1 mM DTT, 5% (v/v) glycerol and 0.5M NaCl.
Source
Escherichia Coli.
Biological Activity
Hemolytic activity for Recombinant Listeriolysin O was 1.18 x105 HU/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay. 2mM DTT is used to reactivate the toxin.
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Related Product Information for LLO active protein
LLO is a single, non-glycosylated polypeptide chain containing 529 amino acids and having a molecular mass of 58kDa. (accession number: AAF64524).
Product Categories/Family for LLO active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LLO hlya (Catalog #AAA10833) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Listeriolysin-O, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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