Human Lymphocyte Adaptor Protein ELISA Kit | Lnk elisa kit

Human Lymphocyte Adaptor Protein (Lnk) (Sandwich ELISA) ELISA Kit

Cat. Num. AAA189903

• Gene Names: SH2B3; LNK; IDDM20
• Reactivity: Human
• Synonyms: Lymphocyte Adaptor Protein; N/A; Human Lymphocyte Adaptor Protein (Lnk) (Sandwich ELISA) ELISA Kit; SH2B3; IDDM20; SH2B Adaptor Protein 3; Lymphocyte-specific adapter protein Lnk; Signal transduction protein Lnk; ELISA Kit for Lymphocyte Adaptor Protein (Lnk); Lnk elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lnk. No significant cross-reactivity or interference between Lnk and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lnk were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lnk were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: 4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year

Standard Curve (Sample)

Related Product Information for Lnk elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Lnk. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lnk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lnk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lnk in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Lnk in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for Lnk elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 611435019
• NCBI GeneID: 10019
• NCBI Accession #: NP_001278353.1
• NCBI GenBank Nucleotide #: NM_001291424.1
• UniProt Accession #: Q9UQQ2; O95184; B9EGG5
• Molecular Weight: 63,225 Da
• NCBI Official Full Name: SH2B adapter protein 3 isoform 2
• NCBI Official Synonym Full Names: SH2B adaptor protein 3
• NCBI Official Symbol: SH2B3
• NCBI Official Synonym Symbols: LNK; IDDM20
• NCBI Protein Information: SH2B adapter protein 3; lymphocyte-specific adapter protein Lnk; signal transduction protein Lnk
• UniProt Protein Name: SH2B adapter protein 3
• UniProt Gene Name: SH2B3
• UniProt Synonym Gene Names: LNK
• UniProt Entry Name: SH2B3_HUMAN