Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/ml
Intended Uses
This LOX-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LOX-1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for LOX-1 elisa kit
Principle of the Assay: LOX-1 ELISAkit applies the quantitative sandwich enzyme ininiunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LOX-1. Standards or samples are then added to the microtiter plate wells and LOX-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LOX-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LOX-1 are added to each well to ''sandwich'' the LOX-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LOX-1 and enzyme-conjugated antibody will exhibit a change in color. The enzynie-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotonietrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,890 Da
NCBI Official Full Name
lectin-like oxidized low-density lipoprotein receptor
NCBI Official Synonym Full Names
oxidized low density lipoprotein (lectin-like) receptor 1
NCBI Official Symbol
Olr1
NCBI Official Synonym Symbols
LOX-1; Oldr1; Oldlr1
NCBI Protein Information
oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density lipoprotein receptor-1; oxidised low density lipoprotein (lectin-like) receptor 1
UniProt Protein Name
Oxidized low-density lipoprotein receptor 1
UniProt Gene Name
Olr1
UniProt Synonym Gene Names
Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
UniProt Entry Name
OLR1_RAT
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Product Notes
The Rat LOX-1 olr1 (Catalog #AAA88706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA88706 ELISA Kit recognizes Rat LOX-1. It is sometimes possible for the material contained within the vial of "Lctin like oxLDL Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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