Human Lipoprotein lipase ELISA Kit | LPL elisa kit
Human Lipoprotein lipase ELISA Kit
Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human
Synonyms
Lipoprotein lipase; N/A; Human Lipoprotein lipase ELISA Kit; LPL (Lipoprotein Lipase)/LIPD; LPL elisa kit
Reactivity
Human
Specificity
Specifically recognize LPL, no obvious cross reaction with other analogues
Sequence Length
475
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for LPL elisa kit
Background: Lipoprotein lipase is a multifunctional enzyme from adipose tissue, heart and skeletal muscle, islets and macrophages. Lipoprotein lipase promotes normal lipoprotein metabolism, delivery and utilization of tissuespecific substrates. Lipoprotein lipase catalyzes the rate-limiting step of lipids in blood circulation.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti LPL antibody was pre-coated onto the 96-well plate. The biotin conjugated anti LPL antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with LPL conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of LPL in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti LPL antibody was pre-coated onto the 96-well plate. The biotin conjugated anti LPL antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with LPL conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of LPL in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,162 Da
NCBI Official Full Name
lipoprotein lipase
NCBI Official Synonym Full Names
lipoprotein lipase
NCBI Official Symbol
LPL
NCBI Official Synonym Symbols
LIPD; HDLCQ11
NCBI Protein Information
lipoprotein lipase
UniProt Protein Name
Lipoprotein lipase
UniProt Gene Name
LPL
UniProt Synonym Gene Names
LIPD; LPL
UniProt Entry Name
LIPL_HUMAN
