Standard Curve (Sample)
Human TNF beta ELISA Kit | LTA elisa kit
Human TNF beta PicoKine ELISA Kit
Gene Names
LTA; LT; TNFB; TNFSF1
Reactivity
Human
Synonyms
TNF beta; N/A; Human TNF beta PicoKine ELISA Kit; Lymphotoxin-alpha; lymphotoxin alpha; Coley's toxin; DAMA-25N12.13-004; DIF; Differentiation inducing factor; Hemorrhagic factor ; LT; LT alpha; LT-alpha; Lta; LTalpha; Lymphotoxin alpha; Lymphotoxin alpha (TNF superfamily, member 1); MGC117668; Natural killer cytotoxic factor; Necrosin; NKCF; OTTHUMP00000037612; OTTHUMP00000037613; TNF B; TNF superfamily member 1; TNF-beta; TNFB; TNFB_HUMAN; TNFbeta; TNFSF 1; TNFSF1; Tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1; Tumor necrosis factor superfamily member 1; LTA elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human LTA
Sequence Length
205
Samples
Cell culture supernatants, serum and plasma (heparin, edta, citrate)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<5 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LTA elisa kit
Principle of the Assay: The Picokine™ Human LTA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LTA with a 96-well strip plate that is pre-coated with antibody specific for LTA. The detection antibody is a biotinylated antibody specific for LTA. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LTA with immunogen: Expression system for standard: E.coli; Immunogen sequence: L35-L205. The kit is analytically validated with ready-to-use reagents. To measure Human LTA, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LTA in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LTA in the sample.
Background/Introduction: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNFbeta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
Background/Introduction: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNFbeta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,297 Da
NCBI Official Full Name
Lymphotoxin-alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1
NCBI Protein Information
lymphotoxin-alpha; LT-alpha; TNF-beta; TNF superfamily, member 1; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
UniProt Entry Name
TNFB_HUMAN
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Product Notes
The Human LTA lta (Catalog #AAA11524) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11524 ELISA Kit recognizes Human LTA. It is sometimes possible for the material contained within the vial of "TNF beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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